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Diabetic cardiomyopathy is a progressive disease in diabetic patients, and myocardial insulin resistance contributes to its pathogenesis through incompletely-defined mechanisms. Striated muscle preferentially expressed protein kinase (SPEG) has two kinase-domains and is a critical cardiac regulator. Here we ...
Data were analyzed using FitMaster (HEKA Instruments) and Prism 6.0 h (GraphPad Software, San Diego, CA). Immediately following recordings, the patch pipette was withdrawn and replaced with a wide-bore (0.2-0.5 MOhm) collecting pipette containing lysis buffer without ERCC mix. Cells were then ...
The ADNI-GO/2 subjects were genotyped using the Illumina HumanOmniExpress BeadChip (Illumina, Inc., San Diego, CA) array [37], while for ADNI-1 subjects, correspondent genotypes were obtained from HumanOmni2.5 BeadChip (Illumina, Inc., San Diego, CA). Quality control was performed using PLIN...
Ultrapure LPS (Escherichia coli 0111:B4) was purchased from InvivoGen (San Diego, CA), murine rIFNg and rIL-4 were purchased from R&D Systems (Minneapolis, MN), murine macrophage colony- stimulating factor (M-CSF) was purchased from PeproTech (Rocky Hill, NJ) and RPMI 1640 medium was ...
"Because we are looking only at medical records, there is a lot we don't know about the mothers and infants in this study," said lead author Dr. Yuyan Shi of the Herbert Wertheim School of Public Health and Human Longevity Sciences at the University of California, San Diego. "But our ...
(Calbiochem, San Diego, CA, USA) for 7 days. After two or three passages in the presence of hygromycin, the cultures were used for experiments without cloning. For bioluminescent tracking, cells were lentivirally infected with a reporter construct encoding green fluorescent protein and firefly ...