myogenic stem cellsUndifferentiated embryonic chick stem cells provide a good in vitro test system to examine the effects of recombinant growth factors on the resultant phenotypic expression of these cells. One of the major difficulties in using freshly isolated cells is variation in the proportion of...
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genom
Abstract. A subset of human regulatory T cells (Tregs) secretes IL-17 and thus resembles Th17 effector cells. How IL-17+ Tregs differentiate from naive pre
( Cancer Sci 2010; 101: 631–638) Stem cells of acute myeloid leukemia (AML) have been identified as immunodeficient mouse-repopulating cells with a Lin − CD34 + 38 − phenotype similar to normal hematopoietic stem cells. To identify the leukemia-propagating stem cell fraction of Philadelph...
Peripheral blood progenitor cells are a prime target for gene therapy approaches. As recent data point to the relevance of soluble stroma factors for the efficient transduction of progenitor cells, we tested the stroma-conditioned medium (SCM) of the two cell lines FBMD-1 and L88/5 as well ...
(n = 3–4 mice per group).cSummary of the frequency and total numbers of ILC2p cells in the BM ofTgfbr2f/fER-Cre+ILC progenitor/CD45.1 BM chimeras post-treated with tamoxifen or oil (n = 3–4 mice per group). Numbers indicate percentages of cells in the indicated gates....
摘要: Cancer Gene Therapy is the essential gene therapy resource for cancer researchers and clinicians, keeping readers up to date with the latest developments in gene therapy for cancer.关键词:Humans Stromal Cells Blood Cells, Cultured Rhodamines Fibronectins Antigens, CD34 Polymerase Chain Reaction ...
However, CD8α+CD11c−Lin−cells failed to differentiate into CD8α− DCs, T cells, natural killer cells, or other myeloid lineage cells in irradiated chimeras. Taken together, all these findings suggest that CD8α+CD11c−Lin− cells are a committed precursor of CD8α+ DCs....
Mast cells originate from hematopoietic stem cells, but the mast cell-committed precursor has not been identified. In the study presented here, a cell population in murine fetal blood that fulfills the criteria of progenitor mastocytes was identified. It is defined by the phenotype Thy-1$^{lo...
The present invention discloses transfected embryonic stem cells capable of providing telomerase positive progenitor cells that are capable of proliferation and maintain