Limiting dilution assay. Methods Mol. Biol., 174: 213-216, 2001.Frisan J,Levitsky V,Masucc M. Limiting Dilution Assay[J].Methods in Molecular Biology,2001,(03):213-216.Frisan, T., Levitsky, V., and Masucci, M. (2001). Limiting dilution assay. Methods Mol Biol 174, 213-216....
网络稀释法 网络释义 1. 稀释法 制稀释法(limiting dilution assay) ,进一步研究T 淋巴先驱细胞的频度是否是造成细胞增殖反应 ir.ym.edu.tw|基于 1 个网页
Alimitingdilutionassayisanexperimentaltechniqueforquantifyingtheproportion ofbiologicallyactiveparticlesinalargerpopulation(Finney,1952;FazekasdeSt. Groth,1982;Taswell,1987).Itisatypeofdose-responseexperimentinwhicheach individualcultureallowsanegativeorpositiveresponse.Replicatesareconducted ...
We developed a tat/rev induced limiting dilution assay to measure the frequency of CD4+ T cells that express multiply-spliced(ms)_SIV RNA in presence and absence of stimulation. We validated the assay using cell lines and cells from blood and lymph nodes of SIV infected macaques. In vitro,...
We have developed a limiting-dilution assay of long-term repopulating hematopoietic stem cells in the mouse using a miniturized stroma-dependent bone marrow culture assay in vitro. The cells were overlaid on irradiated stromal layers in microtiter wells in a range of concentrations, and frequencies...
The analysis of the fraction of nonresponding cultures is performed, as a rule, on the culture fluid. The samples of culture fluid are removed by an automated replicator and deposited on an assay plate. Complement-dependent zones of lysis mark the cultures that have produced antibody. 展开 ...
recognize a subset of 伪6+CD34+ label-retaining cells, and an in vivo limiting dilution assay for keratinocyte stem cells with long-term repopulating ... Anna,S.,Nordvig,... - 《Journal of Investigative Dermatology》 被引量: 10发表: 2012年 Metalloprotease-disintegrin ADAM12 actively promotes ...
A limiting dilution assay is an experimental technique for quantifying the proportion of biologically active particles in a larger population (Finney, 1952, Fazekas de St. Groth, 1982, Taswell, 1987). It is a type of dose–response experiment in which each individual culture allows a negative ...
edu.au/software/elda/ provides an easy user interface.© 2009 Elsevier B.V. All rights reserved.Keywords:Stem cell assaysSingle-hit hypothesisGeneralized linear modelsClopper–PearsonLikelihood ratio testsComputer software1. IntroductionA limiting dilution assay is an experimental technique forquantifying...
摘要: Last month (p. 265) Herman Waldmann and Ivan Lefkovits described the principles underlying limiting dilution analysis. In this article they review some experimental approaches which have been useful in resolving some of the complexities of lymphocyte interactions.关键词:...