Sections from each level were stained with hematoxylin and eosin and for the expression of cytokeratin. The second part of the study examined the relative sensitivity of the light microscope to detect tumor cells in a lymph node. To simulate lymph nodes, cell blocks were made that contained 10...
7373Accesses 23Citations Abstract This chapter is about the physical limitations of optical 3D sensors. The ultimate limit of the measurement uncertainty will be discussed; in other words: “How much 3D information are we able to know?” The dominant sources of noise and how this noise affects...
microscope(SEM) and transmission electron microscope(TEM) observation.Fourier transform infrared spectroscopy(FTIR),ultraviolet and visible absorption spectroscopy(UV-Vis),nuclear magnetic resonance(1H-NMR) and X-ray photoelectron spectroscopy(XPS) analyses demonstrated that the degree of incorporated photo-...
Ultimately this is limited by the inherent wavelike nature of light, to a little under a micrometre. Equations for calculating the Abbe diffraction-limited spot size and the theoretical lateral resolution limit for a given microscope objective and wavelength of light. Surface-enhanced Raman scattering...
Although rare, when analyzed under light microscope, the CISM lesions are difficult to diagnose and there is lack of epidemiological data linked to their biological behavior. A great progress in the diagnosis of these lesions came with the observation that almost all cases of LN and invasive ...
Retinal pigment epithelial cells (ARPE-19) were transduced with cumate-inducible LV expressing vegf-a, with subsequent analysis of vegf-a expression and its impact on cell proliferation, viability, motility, and permeability. Cumate tolerability in adult Wistar rat eyes was assessed as an initial ...
Range of DNA repair in response to double-strand breaks induced in human preimplantation embryos remains uncertain due to the complexity of analyzing single- or few-cell samples. Sequencing of such minute DNA input requires a whole genome amplification t
Simultaneous atomic-resolution electron ptychography and Z-contrast imaging of light and heavy elements in complex nanostructures The aberration-corrected scanning transmission electron microscope (STEM) has emerged as a key tool for atomic resolution characterization of materials, allowing the use of imaging...
For the light microscope observation, they were cut into 1 μ m transverse sections by LKB-5 ultramicrotome 359 (LKB Co., Ltd., Uppsala, Sweden), and stained with 0.5% toluidine blue. Micrographs were captured at a magnification of × 400 with a Nikon Eclipse E600 microscope equipped...
In this work we utilized three different microtiter assays: (1) the colony count, (2), LIVE/DEAD BacLight Bacterial Viability method, and (3) crystal violet staining, to determine the antibiofilm effect of antimicrobials with a diverse mode of action. Using the multidrug-resistantK. pneumoniae...