For quantitative FISH analysis, at least 20 random microscopic fields from several layers were cap- tured for each probing event using a Zeiss LSM 510 Meta confocal laser scanning microscope (Carl Zeiss, Jena, Germany) and image analysis was performed using the program daime. Each probing event...
The purpose of this study was to reveal the regulatory changes ofY. lipolyticain response to nitrogen limitation. Though transcriptional responses to nitrogen limitation have been previously characterized inY. lipolytica[4], the regulatory mechanisms driving these responses are not well understood. We an...
For visualization, a Carl Zeiss LSM 700 Laser Scan Confocal Microscope and the ZEN2009 software (Jena, Germany) were used. In this study, we used primary antibodies directed against C. burnetii, LAMP-1 (Developmental Studies Hybridoma Bank, Iowa, IA, USA), and phosphorylated STAT3 (Cell ...
(i) Time-lapse imaging of Tea4-tdTomato and GFP-Atb2 in wild-type cells shifted to 0.08% glucose for 1 h acquired on the spinning disk confocal microscope. The first six images are maximum projections of two Z-sections. The last image is a projection of the three time points shown ...
lima cells was observed under a laser-scanning confocal microscope (Leica SP8 DIVE/Falcon, Wetzlar, Germany) using the Nile red staining method as described in [19]. Briefly, cells from 50 mL of algal culture under different treatments were collected by centrifugation (3000× g for 5 min at...