Brightfield microscopy normally only provides a low-contrast image of many transparent biological specimens where few details are distinguished. One way to enhance contrast with brightfield microscopy is to use selective stains, but such stains are often toxic to living cells. A phase contrast light...
Because it contains its own light source in its base, a compound light microscope is also considered a bright field microscope. Bright field microscopy simply means that the specimen is lit from below and viewed from above. With bright field illumination, the sample’s contrast comes from its ...
After the pixel super-resolution CNN (SRCNN) was proposed for single-image super-resolution in the field of image processing71, this type of deep learning method was also used in other optical super-resolution problems, such as bright-field microscopy72and fluorescence microscopy73. Similarly, this...
Browse narrower subjects Diode lasers Fibre lasers Free-electron lasers High-field lasers Inorganic LEDs Mode-locked lasers Organic LEDs Quantum cascade lasers Semiconductor lasers Solid-state lasers Ultrafast lasers Browse Lasers, LEDs and light sources across other nature.com journals Pr...
Integrated microscope providing near-field and light microscopy Apparatus are disclosed that perform, on demand, both near- field microscopy (NFM) and light-microscopy (e.g., bright-field microscopy or phase-contrast microscopy) of a specimen. The apparatus comprises an NFM microscope including an ...
在ESI(基本科学指标数据库,Essential Science Indicators,简称ESI) 2024年1月发布的数据中,Light:Science & Applications出版的文章共有221篇文章入选顶尖论文,其中12篇文章入选热点论文,自2023年1月启动顶尖论文发布以来,顶尖论文数持续提高。 基本科学...
High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy H. K. Stelzer, High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy. Plant J. 68... A Maizel,DV ...
Bright-field microscopy and luminescence intensity images of live mouse colon carcinoma cells CT26 (A) and human skin fibroblasts (B) exposed to 2.5 µM P3. Enlarged images are an overlap of the bright-field with luminescence image for the 6 h time point. Ex. 405 nm, reg. 650–750 ...
using optical and transmission electron microscopy. The annealed coupon exhibited a typical austenite single phase, including annealing twins in several grains; the average grain size was 153 μm. After aging heat treatment, κ-carbide was observed within the grains and on the grain boundaries. Addi...
brightfielddarkfielddarkgroundfiltersThe microscope image, especially its contrast, is profoundly influenced by the way that light is directed on to the specimen. The essential controls required are of intensity, evenness, area of illumination and angle of illumination....