descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques Poor access to suitable light-sheet microscopes remains a big obstacle for many end-users in biomedical research. Here, the authors present descSPIM, a low-cost, low-expertise light-sheet ...
image contrast is generated based on scattering and absorbing of the incident light. However, most biological specimens are inherently weakly absorptive and scattering, thus are almost transparent in a conventional bright field microscope. A number of approaches for increasing...
Here, we present a hyperspectral light sheet microscope that acquires spectral information at high resolution for each pixel, while still providing the perpendicular illumination scheme and the low phototoxicity of SPIM. Results Descanned detection. In light sheet microscopy, the sample is illuminated ...
光切法,light-section method 使与被测物体表面成一定角度的平面光束投射到被测物体上,以测定物体表面微观形状、粗糙度等的方法。光切显微镜, light-section microscope 利用光切法测量零件表面粗糙度的仪器。其表面粗糙度测量范围为1～100μm。 水产 光诱围网,purse seine 用灯光诱集捕捞对象后进行包围作业的围网 ...
Show, and The Frozen Planet series. Kinsman is currently an Associate Professor in the school of Photographic Arts and Sciences (SPAS) where he teaches Photographic Instrumentation, Scanning Electron Microscopy, and High-Speed Imaging. His most recent book isCannabis: Marijuana under the microscope...
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Other recommendations for your business Key attributes Other attributes Place of Origin Guangdong, China Brand Name HSD Model Number H-34 Size 10.5x5.3x4cm Product name Children's outdoor exploration microscope Lead time Ratings & Reviews Product reviews(0) ...
FIGURE II.3.8.4.Differential interference contrast on an upright microscope. Light is first linearly polarized, then split by the condenser prism (#1) into two spatially separate beams (shown as solid and dashed). If the two beams do not experience an optical path difference (inset), then the...
cells (Fig.5g); visualization ofz-stack images of E7.5 decidua labeled with SYTOX-Green (Fig.5h); and 3D imaging of E12.0 RARE-lacZ whole mouse embryo (Fig.5i). These data indicate that descSPIM is an affordable and easy-to-build microscope as well as a fully useful microscope. ...
Unlike a stand- ard microscope in SPIM the objective lens is placed per- pendicular to the direction of the light such that the sheet of light illuminates the specimen only at the focal plane of the lens. This has two important benefits; it eliminates scat- tered light from out of focus...