descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques Poor access to suitable light-sheet microscopes remains a big obstacle for many end-users in biomedical research. Here, the authors present descSPIM, a low-cost, low-expertise light-sheet ...
aCAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red.bSize comparison between mesoSPIM v.5 and Benchtop systems.cDetection arm can be equipped with a plan apochromatic objective (2×–20...
1, measured at the exit pupil of a microscope; FIG. 5 shows the diagram of FIG. 4 with a comfortable image brightness drawn in; and, FIG. 6 schematically depicts a stereomicroscope having the transmitted-light base according to the present invention for object examination. DETAILED ...
The idea of Integrated CLEM is equipping a SEM with an inverted fluorescence microscope. Delmic has developed the World’s first integrated CLEM platform, SECOM, which can be retrofitted on any standard SEM,. to perform Integrated CLEM microscopy speedily and easily. Figure 6: A diagram showing ...
We present a physically-based computational model of the light sheet fluorescence microscope (LSFM). Based on Monte Carlo ray tracing and geometric optics, our method simulates the operational aspects and image formation process of the LSFM. This simulated, in silico LSFM creates synthetic images of...
Diatoms often outnumber other eukaryotic algae in the oceans, especially in coastal environments characterized by frequent fluctuations in light intensity. The identities and operational mechanisms of regulatory factors governing diatom acclimation to high light stress remain largely elusive. Here, we identif...
The pumped densities are from 0.35 to 0.59 mJ/cm2, and two mainly emitted peaks at 615 nm and 702 nm are labeled; d integrated intensities of 615 nm and 702 nm emitted peaks and their linear fitting. e Simplified energy-level diagram depicting the energy-transfer process of two-photon ...
A Schematic of the setup that enables simultaneous calcium imaging with two-photon resonance scanning microscope and light stimulus delivery. Light stimuli were pulse delivered during the y-flyback of galvo scanner such that the light does not interfere with GCaMP imaging. B Schematic showing neurona...
lines54. L929 cells were cultured on a fibronectin-coated confocal dish and in DMEM (no phenol red) (Gibco) medium for imaging. During imaging, a microscope incubator system (Tokai Hit, INUF-IX3D-F1) was used to maintain the environmental conditions of 37 °C and a CO2concentration of...
FIGURE II.3.8.4.Differential interference contrast on an upright microscope. Light is first linearly polarized, then split by the condenser prism (#1) into two spatially separate beams (shown as solid and dashed). If the two beams do not experience an optical path difference (inset), then the...