This paper described a homogeneous method, light-initiated chemiluminescent assay (LICA), for quantitation of total testosterone in human sera. The assay was bead based and built on a competitive-binding reaction format, in which 5-伪-dihydrotestosterone (5-伪-DHT) competed with the testosterone ...
Objective To evaluate the analytical performance of light initiated chemiluminescence assay(LICA) for serum α-fetoprotein(AFP). Methods The analytical performance was evaluated with control and calibrator materials,and methodological comparison was carried out with electrochemiluminescence immunoassay using serum...
The active fractions (based on the chemiluminescence assay; see below) were pooled together into two separate stocks to be run on 120 mL Superdex75 column equilibrated with either 50 mM Tris-HCl pH 7.9 or ultra pure water. The active superdex fraction eluted in water were concentrated by...
Stimulation of human neutrophil chemiluminescence by soluble immune complexes and antibodies to neutrophils. Activation of the neutrophil surface membrane may occur with a variety of stimuli, leading to enhanced glucose oxidation via the hexose monophosphate shunt and production of toxic oxygen radicals such...
This review covers advancements in biosensing, biophotovoltaics, and photobiomodulation, focusing on the synergistic use of light, biomaterials, cells or tissues, interfaced with photosensitive dye-sensitized, perovskite, and conjugated polymer organic semiconductors or nanoparticles. Integration of semiconduct...
Species-specific horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) were used, and the signal was developed using enhanced chemiluminescence (GE Healthcare). Blots were exposed to Biomax MR film (Eastman Kodak Co.), and images were prepared for publication using Adobe Photoshop. Protein...
The emitted light was detected and quantified with a chemiluminescence imaging analyzer (LAS3000mini; Fujifilm) and images were analyzed with MultiGauge version 3.0 software. Data Analysis Values are presented as the mean ± S.E., with n indicating the number of animals used; several muscle ...
(HRP) conjugate 1:10,000 (Thermofisher Scientific) and goat anti-rabbit IgG HRP conjugate 1:5000 (Abcam) were used as the secondary antibodies. Normalization of protein content was through VDAC expression. Chemiluminescence quantified with Radiance Chemiluminescent Substrate (Azure Biosystems, Dublin, ...
A SuperSignal™ West Dura Extended Duration Substrate (Thermo Fisher Scientific) kit was used to generate enhanced chemiluminescence from the blotted membranes, which was imaged using a ChemiDoc MP Imaging system (Bio-Rad). Primary antibodies were: rabbit anti-MRPP3 antibody (Abcam, ab185942, ...
Cerebrospinal fluid (CSF) neurofilament light protein (NfL) is a marker of central nervous system neuro-axonal injury. A novel, ultra-sensitive assay can d