The maximum intensity projections of the individual LM channels (DAPI and VWF-FITC, in gray scale) were added to two new layers in the Photoshop file. • Two additional layers were created. One layer was colored green and the other blue using the Paint Bucket Tool (Shift + g). • Th...
Cells were mounted on 20 × 75 × 1.0 mm slide using vectashield (Vector Laboratories, H-1700-2) and sealed with nail polish 1 h later. Nuclear Extraction Cos7-Halo-H2B and Cos7 wild-type cells were grown to ~90% confluency in 100 mm plates. Cells were harvested by...