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(ODE) was added to the flask and the temperature was elevated to 300 °C. A stock solution containing 0.9 mmol of Se dissolved in 2 ml of trioctylphosphine (TOP) was quickly injected into the flask. The reaction temperature was kept for 10 min. Then 0.1 mmol Se dissolved ...
Mutant proteins were generated using a QuikChange II XL site-directed mu- tagenesis kit (Agilent Technologies) according to the manu- facturer's instructions with the following primer pairs: LMTK2(WE477/478AA) 5′-AGCTTTGAGTATGTGG CGGCGGCTGCCAAGCACGAC-3′ and 5′-GTCGTGC TTGGCAGCCGCCGCC...
The concentrations of the protein stock solutions (in 5 mM HCl) were determined with Ellman's reagent. 4.4. XANES and EXAFS X-ray absorption measurements (XANES and EXAFS) were performed at Zinc K-edge using double crystal monochromator [69] at the B18 beamline on Diamond Light Source. ...
The stock solution was used to prepare other five different MB concentrations as: 5, 10, 15, 20, and 25 mg/L. For each concentration, the UV-vis spectra was registered (Figure 14a) and the corresponding absorbance value for the 665 nm band was used to build the calibration curve (...