darkgroundfiltersKöhler illuminationRheinberg illuminationbrightfielddarkfielddarkgroundfiltersThe microscope image, especially its contrast, is profoundly influenced by the way that light is directed on to the specimen. The essential controls required are of intensity, evenness, area of illumination and ...
High-speed three-dimensional (3D) intravital imaging in animals is useful for studying transient subcellular interactions and functions in health and disease. Light-field microscopy (LFM) provides a computational solution for snapshot 3D imaging with low
Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture of information on light spatial distribution and propagation direction...
Various methods of light microscopy are used to enhance contrast and visualize details in unstained tissue preparations. (A) Brightfield microscopy. (B) Phase-contrast microscopy. (C) Differential interference contrast (DIC/Nomarski) microscopy. (D) Darkfield microscopy. ...
Non-isoplanatic lens aberration correction in dark-field digital holographic microscopy for semiconductor metrology 荷兰阿姆斯特丹自由大学的Tamar van Gardingen-Cromwijk教授团队 ① 阅读本文详细解读▼点击下划线文字 全息显微术在半导体测量中的应用 ② 阅读论文原...
The optical pathway, both for the entire wavefront field and a single off-axis light ray, in reflected light DIC microscopy are illustrated in Figures 2(a) and 2(b), respectively. In each case, linearly polarized light from the polarizer is deflected by the half-mirror and enters the Noma...
Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy Xiaoyi Zhu, Qiang Huang, Anthony DiSpirito, Tri Vu, Qiangzhou Rong, Xiaorui Peng, Huaxin Sheng, Xiling Shen, Qifa Zhou,...
electron microscopy- microscopy with the use of electron microscopes dark field illumination,dark ground illumination- a form of microscopic examination of living material by scattered light; specimens appear luminous against a dark background fluorescence microscopy- light microscopy in which the specimen ...
This is in a part of the sky called the Northern Hubble Deep Field that has been observed very deeply at optical wavelengths with HST as well. Since the NICMOS images were only in two bands centered on 1.1 and 1.6 μm, a third band was added, which is a band from previous Wide ...
Light and Color. Illuminators, Filters, and Isolation of Specific Wavelengths. Lenses and Geometrical Optics. Diffraction and Interference in Image Formation. Diffraction and Spatial Resolution. Phase Contrast Microscopy and Dark-Field Microscopy.