To investigate how amino acid sequences of the first heavy chain constant region (CH1) effect on assembly between heavy chain (VH-CH1 or Fd) and light chain (VL-CL), the CH1 gene sequence of an anti-paclitaxel fragment antigen-binding (anti-PT Fab IgG2a) was modified using the splicing ...
However, heavy and light chain assembly occurs rapidly and requires the ATP-dependent release of BiP. We propose that BiP's ATPase cycle is stalled or nonproductive when it is bound to free heavy chains. The binding of light chains to the complex reactivates the cycle and releases BiP....
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Litwin SD, Lin PK, Huitteroth TH, Cleve H: Multiple heavy chain classes and light chain types on surfaces of cultured human lymphoid cells. Nature [New Biol] 246:179-181, 1973Litwin , S. D. , Lin , P. K. , Hutteroth , T. H. , Cleve , H. : Multiple heavy chain classes and...
The in vivo cycling of BiP from heavy chains was monitored using BiP ATPase mutants as kinetic traps. Our data suggest that BiP does not cycle from the C H1 domain of free heavy chains. However, heavy and light chain assembly occurs rapidly and requires the ATP-dependent release of BiP. ...
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3) variable region gene of heavy chain andlight chain 可变区重链和轻链基因4) Chain to chain 链与链5) heavy chain 重链 1. Significance of detecting the clonal rearrangement of immunoglobulin heavy chain gene and T cell receptor γ gene in patients with acute myeloid leukemia; 急性髓性...