网络基于序列连接的化学法 网络释义 1. 基于序列连接的化学法 ...了ABI SOLiD系统,成为投入市场的唯一采用“基于序列连接的化学法”(ligation-based)的测序平台,在去年的全球基因组测 … www.labbase.net|基于6个网页
One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded ...
There are two main DNA fragmentation methods for ligation-based library prep: Physical—When DNA is physically sheared using acoustics, nebulization, centrifugal force, needles, or hydrodynamics. While these methods achieve accurate, unbiased results, they require specialized machinery. For example, genomi...
Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers Molecular diagnosticsrolling circle amplificationRCAepoxymicrofluidicslab-on-a-chipOSTEoff-stoichiometry thiol-eneFor the first time, we demonstrate DNA mutation ... F Saharil,A Ahlford,M Kuhnemund,.....
Pandey V, Nutter RC, Prediger E: Applied Biosystems SOLiDTM System: Ligation-Based Sequencing. In Next Generation Genome Sequencing: Towards Personalized ... MULLIN,RICK - 《Chemical & Engineering News》 被引量: 1发表: 2011年 A high-resolution, nucleosome position map of C. elegans reveals a...
Based on this assumption, we developed Refresh-seq (restriction fragment ligation-based genome amplification and TGS), a novel single-cell long-read whole-genome sequencing technique based on restriction endonuclease cutting and ligation strategy (Fig. 1 and Supplementary Fig. S1a). Restriction ...
(NGS) have increased its potential for disease diagnoses, clinical oncology, variant detection, comparative genomic profiling, new genome assembly, and exploratory research. Among the many necessary components, sequencing adapters are critical for NGS in ligation-based workflows. This article will cover ...
We have developed a fast, simple, and efficient ligation-based single-stranded DNA library preparation method engineered to produce complex NGS libraries from one nanogram of DNA without altering the native ends of template molecules. Our method, called SRSLY (Single-Reaction Single-stranded LibrarY)...
Here, we demonstrate the efficacy of a novel, high-throughput, ligation-based assay for simultaneous quantitative detection of multiple plant pathogens. The ligation probes, designated Plant Research International-lock probes (PRI-lock probes), are long oligonucleotides with target complementary regions ...
Oligonucleotide ligation assay- based DNA chip for multiplex detection of single nucleotide polymorphism. Biosens Bioelectron 2004; 19: 1277-83.Deng JY,Zhang XE,Mang Y,et al.Oligonucleotide ligation assay-based DNA chip for multiplex detection of single nucleotide polymorphism. Biosensors and ...