虽然任何dsDNA都可以用作连接测序试剂盒(Ligation Sequencing Kit)的模板,但如果用户计划定期对扩增子或cDNA进行测序,应考虑使用附带有专用方案的特定试剂盒,以简化实验室工作流程并获得优质的结果。如果使用扩增子,推荐联合使用我们的四引物PCR实验方案(four-primer PCR protocol)和PCR扩增测序试剂盒(
No input .fast5 was found or basecalledinput_pathdid not point to the .fast5 file locationThe--input_pathhas to be followed by the full file path to the .fast5 files to be basecalled, and the location has to be accessible either locally or remotely through SSH. ...
Unusually slow processing on a GPU computerTheflag wasn't included in the commandTheflag specifies a GPU device to use for accelerate basecalling. If not included in the command, GPU will not be used. GPUs are counted from zero. An example is--device cuda:0 cuda:1, when 2 GPUs are sp...
No input .fast5 was found or basecalledinput_pathdid not point to the .fast5 file locationThe--input_pathhas to be followed by the full file path to the .fast5 files to be basecalled, and the location has to be accessible either locally or remotely through SSH. ...