Inactivate T4 DNA Ligase by heating the reaction mixture at 65℃ for 10 minutes. The resulting ligation products can be digested directly with restriction endonucleases.
T4 DNA Ligase, Blue/White Cloning Qualified ProtocolPDF(112 KB)– English Specifications You are viewing:M1801Change Configuration What's in the box? ItemPart #SizeAvailable Separately T4 DNA Ligase 10X Buffer C126B1 × 200μlView Product ...
Frilander, M. J., and Turunen, J. J. (2005) RNA Ligation Using T4 DNA Ligase, Wiley-VCH, Hoboken, New JerseyFrilander MJ, Turunen JJ (2005) RNA ligation using T4 DNA ligase. In: Hartmann RK, Bindereif A, Schon A, Westhof E (eds) Handbook of RNA biochemistry. WILEY-VCH Verlag ...
Backbone and insert were ligated using T4 DNA ligase (New England BioLabs, #M0202). NEB 5-alpha competentEscherichia coli(high efficiency) cells (New England BioLabs, #C2987I) were transformed with the ligated plasmid. Transformed bacteria were plated on LB + Amp (100 µg/mL) agar...
T4 DNA ligaseunnatural base pairWe present cognate base pair selectivity in templateヾependent ligation by T4 DNA ligase using a hydrophobic unnatural base pair (UBP), Ds㏄a. T4 DNA ligase efficiently recognizes the Ds㏄a pairing at the conjugation position, and Ds excludes the noncognate ...
Here we describe LUMI-PCR (Ligation-mediated Unique Molecular Identifier PCR), a protocol which integrates Illumina dual index library construction with splinkerette based ligation-mediated PCR. The adapters are compatible with a standard Illumina dual index sequencing recipe and can be used to estimate...
2×ligation solution MasterMix是含有T4 DNA Ligase,ligation buffer的2×MasterMix,实验时,只需加入片段和载体即可进行连接反应。适用于DNA片段和载体DNA的连接以及DNA片段和Linker或Adaptor DNA的连接。按照10μl连接体系计算,每次使用5μl,可以使用30次。
英文名称:T4 DNA Ligase(with 10X Ligation Buffer) 英文同义词:T4 DNA Ligase(with 10X Ligation Buffer) CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览T4 DNA Ligase(with 10X Ligation Buffer)国外供应商中国供应商 T4 DNA Ligase(with 10X Ligation Buffer)推荐供应商 ...
Hieff NGS®UltimaDNA Ligation Module是针对Illumina®与MGI®高通量测序平台文库构建专业设计的DNA连接模块,其中最大的亮点为本产品采用一款我公司自主研发的高效连接酶Fast T4 DNA ligase。本产品可在双链平末端DNA 片段或双链3′-dA DNA 片段的两端连接Illumina®与MGI®测序平台适用的DNA接头,具有连接效率...
A novel approach to building a limitless variety of mobile multiple-restriction DNA fragments termed “uni-linkers” and which are open only at one end, is described. The latter were used as “multi-stage” linkers in cloning experiments. The cloning of the cDNA for a carrot proline-rich ...