Cell culture and stimulation AC16 human ventricular cardiomyocytes were cultured in complete DMEM (Life Technologies Corporation, Carlsbad, CA, USA) with 10% FBS (omega scientific, Tarzana, CA, USA) and 1% Antibiotic–Antimycotic (Gibco, Grand Island, NY, USA). At 80% confluency, cells were ...
For supply chain continuity, Life Technologies manufactures DMEM/F-12 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers...
Polymerase chain reaction (PCR) was performed with Fast Start Universal SYBR Green Master (Roche), and fluorescence was measured using the Step-One Plus realtime PCR system (Life Technologies Applied Biosystems, Grand Island, NY). The following primer sets were used: Reelin (NM_011261, sense 5...
The resulting dried precipitated DNA was then resuspended in HiDitm formamide (Life Technologies, Grand Island, NY, USA), heat-denatured and sequenced on the departmental ABI 3130xl Genetic Analyzer at UL Lafayette. ®DNA assembly and analysis. The resulting sequences were then assembled on ...
protocols on ABI3730XL machines at the HudsonAlpha Institute in Huntsville, AL, USA, and the JGI according to the manufacturer’s specification (Life Technologies, Grand Island, NY, USA). The assembly was generated using Newbler version 2.6 with default parameters after trimming poor bases from ...
For supply chain continuity, Life Technologies manufactures Gibco® F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device ...
Most of the arguments for not using these technologies are virtually the same which were heard 45 years ago when planning began to build the Baltimore ERUV. Many decried how Shabbos would be altered; people would forget the halachos etc. THEY WERE RIGHT!!! As a young boy, part of my Er...
Subsequently, the libraries were checked using an Agilent 2100 bioanalyzer and DNA High Sensitivity Ki t (5067-4626, Agilent Technologies, Inc., Santa Clara, CA, USA) to evaluate the mean fragment size. Quantification was performed using the library quantification kit for the StepOne Real-Time ...
For supply chain continuity, Life Technologies manufactures Gibco® F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device ...
(CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Life Technologies offers a variety of Gibco® F-12 modifications for a range of cell culture ...