resuspended in 200 mmol/l NaHCO3(pH 8.4) and labeled for 15 min with Alexa633-NHS (Life Technologies, Darmstadt, Germany) at a final concentration of 2 μmol/l. Labeling reaction was quenched
DYRK1B and STAT3 were cloned in frame with C and N-terminal NanoLuc or HaloTag, respectively. For the transfections, HEK293T cells were diluted in OptiMEM without phenol red (Life Technologies) to 4 × 105cells/ml and 38 ml were pipetted into white 384-well plates (Greiner). 1 ...
The odorants used were 3-mercapto-2-methylpentan-1-ol (#242803), 3-mercapothexyl acetate (#137912, Chemos GmbH, Regenstauf, Germany), 2-phenylethanethiol (#P1715, TCI Deutschland GmbH, Eschborn, Germany), allyl phenyl acetate (#W203904), and (R)-(-)-carvone (#124931, Sigma-Aldrich...
Julia SchützLCEE Life Cycle Engineeering Experts GmbH Birkenweg 24 Darmstadt GermanyTorsten MieleckeLCEE Life Cycle Engineeering Experts GmbH Birkenweg 24 Darmstadt GermanyPeter SalingCarl‐Bosch‐Strasse BASF S.E. Ludwigshafen GermanyJohn Wiley & Sons, LtdCivil Engineering Design...
Dissertation, TU Darmstadt, Darmstadt (2020) Google Scholar [10] Mapal Dr, KG. Kress Lasersintern erweitert Fertigungsmöglichkeiten von Präzisionswerkzeugen Diamond Business (2017) Google Scholar [11] Karl-Heinz Arnold GmbH Florett statt Bazooka WB Werkstatt + Betrieb No. 3 (2021), pp...
All image analysis was done using PFUS v4.0 (PMOD Technologies GmbH, Zurich, Switzerland). Following PET/CT imaging, mice were euthanized with a Dolethal® (200 mg/mL pentobarbital) (Vetoquinol, Niel, Belgium) overdose (350 mg/kg) injected intraperitoneally. After transcardial perfusion with ...
mounted 90 cm above the arena and connected to a PC via PCI interface serving as frame grabber for video tracking software (Video Mot2 software, TSE Systems GmbH, Bad Homburg, Germany). Exploratory behavior in the open field Pre-juvenile rats (P17) were allowed to freely explore the ...
Integrating Life-Cycle Assessment into Automotive Manufacturing - A Review-Based Framework to Measure the Ecological Performance of Production Technologies H.C.C.F. Schebek (Ed.), Progress in Life Cycle Assessment, Sustainable Production, Life Cycle Engineering and Management, Springer, Cham (2018),...
(Stem cell technologies, Cologne, Germany). When cells were 70–80% confluent, they were transfected with XLone TRPC6-YFP plasmids using Lipofectamine 3000 (Invitrogen, Darmstadt, Germany) according to the manufacturer’s manual. A total of 500 ng plasmid DNA containing PiggyBac (PB) ...
The central goal of the innova- tion alliance is to develop technologies and methods for digitalizing the process- es and for gathering and processing infor- mation. The intention is also to promote the use of the data that has been acquired in production and recycling processes. At the ...