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Cells medium was removed and metabolites were immediately extracted in 2 mL ice-cold (− 20 °C) 80% MS-grade MeOH (Ref. 900688; Sigma-Aldrich) in sterile pyrogen free water Otec (Ref. 600500; Aguettant; France). After vortexing for 1 min, samples were stored at − 80 °C before...
General freedom of action is an emanation of human dignity and the free development of the personality; at times referred to as the incarnation of liberty, however, not often expressly implemented in constitutions. The right to life and the protection of physical and mental integrity are the most...
Identification of candidate genes associated with tubal origin of high‑grade serous ovarian cancer Xiang L, Rong G, Zhao J, Wang Z, Shi F Oncology Letters 2018 vol: 15 (5) pp: 7769-7775 The protective autophagy activated by GANT-61 in MYCN amplified neuroblastoma cells is mediated by PER...
(PVDF) membrane at the bottom. Protein digestion was performed with sequencing-grade modified trypsin (V5111, Promega; Madison, WI, USA) at a nominal protease to protein ratio of 1:25 w/w. After incubation for 2 h at 37 °C, the peptides were eluted from the PVDF membrane, and ...
RENEE MONTAGNE
NotesHumanThe COVID-19 pandemic, a crisis never seen before in human history, took the world by surprise, and pandemic prevention and control measures therefore become a common feature of the social and political lives of all countries, including China. Obviously, prevention and control of the ...
, and an additional ~ 30 kDa LRP10 species was only detected in cell lysates from the patient P1 and P2 astrocytes (Fig.6E, cell lysates, pink arrowheads). This observation is in line with the predicted size of the truncated protein that results from the mutant allele. Conditioned ...
single band. Gel bands were excised, reduced with 20 mM dithiothreitol at 56 °C, alkylated with 55 mM iodoacetamide at RT in the dark and digested with mass spectrometry grade trypsin (Promega, Germany). Trypsin was added at a ratio of 1:30 with respect to the total protein content of...