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Department of Chemistry and Life Sciences, University of Aalborg, Denmark M. von Bergen Contributions K.H., H.S., S.D. and J.R. are responsible for performing the underlying animal experiment and the creation of all none-metabolomic data. U.R.-K. performed the sample preprocessing and LC...
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10 mM iodoacetamide for 30 min in the dark at 25 °C. After quenching the reaction with 12 mM N-acetyl-cysteine the samples were diluted with 100 mM ammonium bicarbonate buffer to a final DOC concentration of 1%. Proteins were digested by incubation with sequencing-grade modified ...
rna circpbx3 promotes cisplatin resistance of ovarian cancer cells via interacting with igf2bp2 to stabilize atp7a mrna expression. hum cell 35:1560–1576.https://doi.org/10.1007/s13577-022-00748-8 li h, luo f, jiang x, zhang w, xiang t, pan q, cai l, zhao j, weng d, li y ...
biochemical reactions and by impairing energy generation via disruption of adenosine triphosphate (ATP) production. The toxicity of trivalent arsenite [As(III)] is mainly attributed to its high affinity for sulfhydryl groups, and binding of As(III) to reduced cysteine residues in proteins may ...
The progression of cancers and neurodegenerative disorders is largely defined by a set of molecular determinants that are either complementarily deregulate
(10 µL 1 M Tris–HCl pH 7.5 + 2 µL proteinase K PCR Grade 20 mg/mL and H2O to make 1 mL) was then added to the tissue samples, incubated for 2 min at 4 °C, and the slides were rinsed with PBS. As the positive control, fixed and permeabilized samples were incubated...