Guide oligonucleotides (Sigma-Aldrich) were introduced into theC. parvumCas9/U6 plasmid16by restriction cloning. See Pawlowic et al.48for a detailed discussion of guide design forC. parvum. Transfection plasmids were constructed by Gibson assembly using NEB Gibson Assembly Master Mix (New England B...
ScreenLife Capture is an open-source Android application which comes with a companion DMPO software. Figure1provides an overarching framework of the workflow and how screenshots are captured, transferred, and processed before analysis. As detailed below, there are three main stages to the workflow p...
The brick production phase consisted of both the mixing and heat-curing processes stated in Table 2. Table 2. Detailed life cycle phases considered for the analysis. 3.2. Raw Material Acquisition The brown coal ash was obtained from two power plants in the La Trobe Valley, Victoria, ...
However, the results were not significant, which allowed maintaining the environmental impact assessment processes and proposing new analysis strategies, such as life cycle assessment for water quality studies and decision making. This criterion is related to agricultural activities and urban water ...
Here, we demonstrate that these convergent advances now enable the generation of brain charts that (1) robustly define normative processes of sex-stratified, age-related change in multiple MRI-derived phenotypes; (2) identify previously unreported brain growth milestones; (3) increase sensitivity to ...
of the supply chain. By looking across the entire supply chain, it is possible to optimize the use of chemicals and energy so that only those chemicals having the fewest impacts, together along with the most appropriate chemistries, synthetic strategies, and the best possible processes are ...
PeopleSoft Payables: This application processes invoices for payment and posts the results in PeopleSoft GL based on the templates and account coding. The drill back and drill forward actions support these flows: Drill back from ledger entry to revenue recognition transactions for information about re...
Over the last few years, studies in humans have started to provide a detailed understanding of somatic mutation rates and the contribution of endogenous and exogenous mutational processes across normal tissues8,13,14,19,20. These studies are also revealing how, as we age, some human tissues are...
Brand notes that building alteration decisions are usually based on the slower-changing layers (e.g. structural capacity), but occasionally a faster-changing layer causes major alterations because it cannot be modified independently (e.g. installing heavy equipment requiring structural strengthening). ...
The core of this procedure is a standard Service Broker receive loop that receives messages one at a time and processes them based on the message type. The EndConversation and Error message types are handled by standard Service Broker routines. The TaskRequest message type starts SQL tasks. The...