After dissection of hearts, cells were dissociated by repeated enzymatic digestion (0.08% trypsin in PBS, GIBCO 15090046; plus 50 μg/ml DNaseI, Roche 10104159001) and cultivated in DMEM, high glucose, GlutaMAX supplement (GIBCO, 31966021) supplemented with 10% fetal calf serum (GIBCO, 10270-...
performed using the Anti-Collagen IV primary antibody (Anti-Collagen IV, Abcam: ab6586, 1:500°) and a secondary Alexa488 Fluor-conjugated antibody (1:500; Molecular Probes, Invitrogen) and DAPI (ACDBio) was used to stain the nuclei. Sections were analyzed using an Axio Imager.Z2 ApoTome...
GLUTAMAX I CTS TM 100X,产品货号|编号:Invitrogen-A1286001,价格及说明书详询!Invitrogen/life technologies(Gibco,Ambion,ABI,Biosource,MP bio,Zymed,Caltag,Dynal) KNOCKOUT TM DMEM CTS TM,产品货号|编号:Invitrogen-A1286101,价格及说明书详询!Invitrogen/life technologies(Gibco,Ambion,ABI,Biosource,MP bio...