Characterization of a cDNA library, enriched for fragments of genes preferentially expressed in the embryonic day 14.5 mouse forebrain as compared to the adult forebrain, was performed using DNA sequencing and in situ RNA hybridization to sections of mouse embryos. One hundred and thirty different ...
Creating a DNA Library A DNA library contains as many genes from the organism of interest as possible. The genomic DNA from the organism of interest is isolated and digested with a restriction enzyme. Usually, the restriction enzyme used has a recognition sequence of four base pairs; therefore,...
In addition, using a single DNA probe to screen the traditional library is a simple precursor to the common procedure of using a panel of probes to do whole exome sequencing. After cloning all the possible genes from an organism into a library, the next step is to identify the genes of ...
was confirmed by co-transformation of protoplasts with the individualAvrgene candidates and the correspondingRgenes, which resulted in a significant reduction in YFP-positive cells compared with theRorAvrgenes alone or non-matchingR–Avrgene pairs (Fig.3a). Similarly, specific recognition was also ob...
For next generation DNA sequencing, we have developed a rapid and simple approach for preparing DNA libraries of targeted DNA content. Current protocols for preparing DNA for next-generation targeted sequencing are labor-intensive, require large amounts of starting material, and are prone to artifacts...
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
The availability of the CHORI-321 American mink BAC library will aid in identification of genes and genomic regions of interest. We have demonstrated how the library can be used to identify specific genes of interest, develop genetic markers, and for BAC end sequencing and deep sequencing of sel...
Demonstration of an actual screening experiment Learn about a library screening we performed from start to finish to identify the genes involved in 6-thioguanine (6-TG) resistance. Also, see sequencing data and the description of useful basic analysis tools. Watch Now...
286 MATERIALS AND METHODS (a) Gene library, molecular cloning and preparation of RNA The wheat-Charon 4 gene library, molecular clon- ing of genes, and preparation of whole nuclear RNA from germinated wheat seeds were described pre- viously (Tabata et al., 1983). (b) Nucleotide sequencing ...
Simple, cost-effective chromatin immunoprecipitation sequencing (ChIP-Seq) DNA library preparation, with master mixes and robust multiplex capabilities.