Here, by leveraging massively parallel plasmid-cloning methodology, we show that arrayed libraries can be constructed for the genome-wide ablation (19,936 plasmids) of human protein-coding genes and for their activation and epigenetic silencing (22,442 plasmids), with each plasmid encoding an array...
The invention is directed to methods for the creation and use of libraries of proteins which comprise polyclonal antibodies to a common antigen or group of antigens, receptor proteins with related variable regions, or other immune related proteins with variable regions. These polyclonal antibody librar...
The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also
and the antigen. Moreover, the display level of a cloned Nanobody on the surface of an individual yeast cell can be monitored through a covalent fluorophore that is attached in a single enzymatic step to an orthogonal acyl carrier protein (ACP). Additionally, the displayed Nanobody can be ...
By using the repurposing strategy, many compounds have already been identified as new potential drugs against several diseases, including parasitosis, protozooses, and mycoses (45, 47–52). Here, we screened two chemical collections to analyze A. fumigatus in vitro susceptibility to compounds ...
Thus, where protein function restoration is required, the host cells will typically be derived from cancerous or other diseased cells and naturally produce the disfunctional target protein. Suitable cell lines are available, for example in cell culture collections such as the ATCC and may be ...