将EGFP-IRES-creERT2-WPRE-polyA插入到小鼠Lgr5基因起始密码子处。 应用领域:Cre工具鼠,Lgr5 可作为肠和胃的干细胞的标志基因。表达细胞类型包括胃腺上皮干细胞、肠的隐窝细胞和毛囊干细胞等。 *使用本品系发表的文献需注明: Lgr5-EGFP-IRES-CreERT2 mice (Cat. NO. NM-KI-200154) were purchased from Sha...
Lgr5-creERT2品系在小鼠自身Lgr5基因终止密码子后,插入由IRES衔接的creERT2基因。IRES衔接的creERT2基因与小鼠Lgr5基因共转录,同时creERT2基因仅在Tomoxifen诱导下表达cre蛋白。 Lgr5-creERT2小鼠可作为组织特异性范围内诱导LoxP重组的Cre工具鼠。例如删除FLOX区域,即将该工具鼠与得到的条件性敲除模型小鼠配繁,在...
作者为了可视化活的CBC细胞并研究它们潜在的"干性",通过将增强的绿色荧光蛋白(EGFP)-IRES-creERT2基因box在第一个ATG codon敲除等位基因,并以此作谱系追踪。异质小鼠是健康和肥沃的。在成人组织中观察到的 GFP 模式忠实地概括了以前看到的Lgr5-lacZ 等位基因模式(未显示的数据)。通常,CBC细胞在基座相对较宽,扁平的...
We performed embryonic lineage tracing in Lgr5-GFP-IRES-CreERT2 mice. Results We show that LGR5 is part of the human definitive endoderm (DE) gene signature, and LGR5 transcripts are induced robustly when human pluripotent stem cells are differentiated into DE. Our results show that LGR4 ...
综上所述,本研究描述了一种可以有效且选择性地针对胃肠道内胃上皮的所有隔室进行基因重组的Cldn18-IRES-CreERT2等位基因,利用在此基础上构建的晚期GC模型,确定了TR-Lgr5+SC对体内GC的发生、进展和转移具有重要贡献,突出强调了开发靶向...
Intestinal tissues from these mice, as well as Apc+/Min and ApcCKO/CKO/Lgr5-EGFP-IRES-CreERT2 mice, were analyzed by immunohistochemistry and in situ hybridization. Results We identified promoter regions of 960 genes that interacted with the Wnt pathway nuclear effector T-cell factor 4 in 4...
PUFA and curcumin action in the AOM-induced colon cancer model. Unfortunately, the percentage of GFP+Lgr5 cells within the ACF could not be determined due to the mosaic nature of expression in the Lgr5-EGFP-IRES-creERT2knock-in mouse,25that is, a large fraction of ACF were GFP negative...
The file contains Supplementary Figures 1-4 with Legends. In Supplementary Figure 1 it is shown that GFP-positive CBC cells in the small intestine of LGR5-Ires-CreERT2 knock-in mice are frequently Ki67 positive, confirming that they are typically cycling cells. The general strategy used to ge...
Culture of Lgr5–EGFP–ires–CreERT2 crypts revealed Lgr5–GFP 1 stem cells intermingled with Paneth cells at the crypt base. Wnt activation, as demonstrated by nuclear b-catenin (Supplementary Figs 4a and 9) and expression of the Wnt target genes Lgr5 (Fig. 1d) ...
and the turnover rate of epithelial cells was accelerated significantly afterBmpr1ainactivation (Supplementary Fig. 1g). To examine whether loss of BMP response in Lgr5+ISCs is responsible for their expansion, we deletedBmpr1aspecifically in these cells usingLgr5-EGFP-IRES-CreERT2;Bmpr1afl/fl(Lg...