可得到以约32∶1的摩尔比偶联到BSA上的合成Lewis Y四糖(Alberta Research Council,Edmonton,Alberta,Canada)。用Lewis Y-BSA抗原(50μl,3.0μg/ml PBS)包被ELISA板(Nunc MaxiSorp),并在4℃温育过夜。用3%BSA/PBS在室温下阻断板上的非特异性结合2小时。将半对数连续稀释的样品(上清液或纯化的抗独特型mAbs)加入...
In vitro binding to Lewis-Y and protein A of hu3S193 mutant antibodies The Lewis-Y binding activity of hu3S193 antibodies was determined by BIAcore analysis using a synthetic Lewis-Y tetrasaccharide coupled to BSA (Alberta Research Council, Edmonton, Alberta, Canada) on a CM5 chip using a BI...