The protein was sealed at room temperature for 2 h with 5% skimmed milk powder and then incubated with each of the following antibodies for overnight at 4℃: rabbit anti-RCAN1 (1:500, T73889, Abmart), rabbit anti-IGF1 (1:500, PA3635, Abmart), rabbit anti-DHCR24 (1:1000, TD12472...
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Protein powder supplementation in early pregnancy and the risk of gestational diabetes mellitus: a prospective cohort study. Food Funct. 2023;14(8):3815–23. Article CAS PubMed Google Scholar Riskin-Mashiah S, Younes G, Damti A, Auslender R. First-trimester fasting hyperglycemia and adverse ...
15, Pages 5852-5873: Nanoparticle-Mediated Pulmonary Drug Delivery: A Review Colloidal drug delivery systems have been extensively investigated as drug carriers for the application of different drugs via different routes of administration. Systems, such as solid lipid nanoparticles, polymeric nanoparticles...
The aim of this study is to investigate the viability of performing initial artificial joint replacement surgery in patients presenting with unexplained elevations in erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) levels. A cohort of 2
Annual Review of Plant Physiology and Plant Molecular Biology 49, 611–641 (1998). 3. Dolch, L. J. & Marechal, E. Inventory of fatty fcid fesaturases in the pennate fiatom Phaeodactylum tricornutum. Mar Drugs 13, 1317–1339 (2015). 4. Joyard, J. et al. Chloroplast proteomics ...
Protein and RNA extraction and RT-qPCR analysis (Cohort #2) Approximatively 50 mg of this fine powder were used for total protein extraction. Then, a lysis buffer (50 mM Tris–HCL to pH 7.4, 150 mM NaCl, 1% triton and 0.5% sodium deoxycholate) containing protease (complete 25 X and Pe...
96-well MaxiSorp ELISA plates (Nunc, Cat No. – 442404) were coated with 50 µl of 1 µg/mL purified N protein diluted in 1X PBS pH 7.4 and the plates were incubated for 1 h at room temperature (RT). Serum samples were inactivated by adding 10 µl of 10% Triton ...
Anaesthetics have an impact on cell homeostasis especially through modulation of protein post-translational modifications. O-GlcNAcylation, a ubiquitous post-translational modification, plays a role in many biological processes. The aims of this study were to evaluate whether (1) anaesthesia influences O...
(systemic blood pressure and pulse rate), self-reported adverse events, blood cell count, electrolytes (Na, K, Cl), kidney function (creatinine), liver function (aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, and bilirubin), inflammation (C-reactive protein), ...