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RN732ATTD26R1B50、G86305111TEU、KTR18EZPJ362、ERJ-P06D2260V、V10E20P、345-116-523-201、AFC475M50C12B-F、NMP1K2-KCCCCC-07、EB13E2D2H-1.8432M、RN73C1J931RBTG、T100-001-TST、AT4127SF、62_4310-U0-0-6/---_-Y、XMLBWT-02-0000-000HT30F7、PLA1G221J05、UP2.8B-270-R、MKP4R03...
CCAAGGTAGTCGGCAAATAATTCCCCGGTTTTTGAATTGTAGC Sigma-Aldrich pUC19_GltB_KO_DS_fw CGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGGGGCGGTTGATTACCTTCCTGC Sigma-Aldrich pUC19_GltB_KO_DS_rev CAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGCTCGAGATGGTAACGCTACTCGAAAATC Sigma-Aldrich G6PDH fw ol pET15b CCAACTCAGCTTCCTTTCGGG...
Annealed oligo cloning with the 5′-phosphorylated Primer 3 (TCG AGA ACC TGT ATT TTC AGA GCG) and 4 (TCG ACG CTC TGA AAA TAC AGG TTC) was used to introduce the specific recognition sequence of the TEV protease into the XhoI restriction site of pET23a(+)_wbgU6xhis. Consequently, the ...
(5'-GGCGAATTCTTTAATTAGAAGTAGGAGGTATCGAC-3'). The underlined region denotes theEcoRI restriction site. The signal peptide sequence of a putative PME (At4g12390) fromArabidopsiswas PCR amplified from BAC clone T4C9 (supplied by ABRC) using the forward primer OVEXP1 (5'-GGCGGATCCTTATGGAACCA...
STMN1-specific siRNA oligos (STMN1 siRNA1; GAAACGAGAGCACGAGAAAtt: STMN1 siRNA2; CGAGACUGAAGCUGACUAAtt) and a non-targeting control siRNA oligos (NT siRNA) were purchased from Bonac Corporation (Fukuoka, Japan). MKN7 and MKN45 cell lines were seeded at 1 × 105 cells per well in a...
As mentioned above, stimulation of growth gai(NwbnEmryhHo-pibeNwc4rli+heenHevnvi4fauitse+otnrec)cutdodowislneniCerezdsrxaiOieesttpltii2eseeooivnrwnngiatsnm;at,weisafewditimnctihCatttoshnhO8ast.ettl2ChyFcseguOlgaeermrla2ntoe-hraeewilnetyreeradtsrmmilutmlytcoahleeenrha,dveinae,ftdeilhdtns...
σc = T t αt ,c h2t ,c −µ2c (9) wis hthereeaσcctiivs atthieonweviaglhuteedatsmtaonmdaerndtdte .vTiahteiofnin, aαlto,cuistpthuet osefltfh-aettpeonotilvinegscloayree,rµisc is the weighted mean vector obtained by connecting the avnecdthotr,sc of weighted mean ...
isdhnifotitnhgatvheeaanpypsairgennitfiTcamnvtaelfufeecftr(oSmup7p2l.e9m meninta(rWy FTi)g.t oS1634a.5). mThine creosnuslttrsuwcterheaorbbtoariinnegdaflolrtmhreeme bmruantaetisoanmsp(l3e-sMfrUomT)Eexxphi2ib9i3teFdceallTsm(nvoatlusheoswimn)i.laMr otoditfhicaattioofnts...
TThhee ffiirrsstt ssttaaggee iinn Eqquuaattiioonnss ((1199)) aanndd (200)) determines the minimum daily peak demand that can be achieved, while the second stage minimises the energy charged to achieve the minimum daaiillyy ppeeaakk ddeemmaanndd.. The daily peak demand K in Equation (...