After dehydration in an alcohol gradient series and propylene oxide (Merck), the tissue samples were embedded in glycid ether 100 (Serva, Heidelberg, Germany). Ultrathin sections were cut on a Leica ultramicrotome (Leica Ultracut S, Vienna, Austria), stained with uranyl acetate (Sigma–Aldrich)...
This Game contains content that may not be appropriate for all ages, or may not be appropriate for viewing at work: Frequent Violence or Gore, Depictions of Drugs and Alcohol, General Mature Content 系统需求 最低配置: 操作系统 *:Windows 7, 10, 11 ...
Immediately prior to drug injection, the site was wiped with 70% isopropyl rubbing alcohol and allowed to air dry; the drug was administered sc using a 3 ml disposable luer-lock syringe with 25-gauge 5/8-inch needle. At each injection site, injection volume was ~ 2–3 ml. ...
The effects of acetyl fentanyl can be potentiated by other central nervous system (CNS) depressants, including other opioids. When it is used with alcohol, synergistic effects occur. Benzodiazepines also have an additive and possibly synergistic effect on CNS and respiratory depression.11 When these ...
Institute of Veterinary Medicine at the Warsaw University of Life Sciences—SGGW, Poland, for histopathological examination. The samples were routinely processed: dehydrated in increasing gradients of ethyl alcohol, embedded in paraffin and cut into 4 µm-thick sections using a microtome. Subsequently...
Previously we have shown that normally nonlethal doses of hepatotoxicants, such as allyl alcohol, CCl 4 , and thioacetamide (TA), cause 80 to 100% mortality in type 2 diabetic (DB) rats indicating hepatotoxic sensitivity. Even though liv... SP Sawant,AV Dnyanmote,HM Mehendale - 《Faseb ...
In brief, 500 μL of extraction buffer (200 mM Tris (Roth), 20 mM EDTA (Roth), 200 mM NaCl (Roth), pH 8.0), 200 μL of 20% SDS (AppliChem), 500 μL of phenol:chloroform:isoamyl alcohol (PCI) (24:24:1) (Roth) and 100 μL of zirconia/silica beads (0.1 mm diameter) (...
By testing an acute oral dose of the fungicide product Amistar, and equivalent doses of each individual co-formulant, we were able to measure the toxicity of the formulation and identify the ingredient responsible. We found that a co-formulant, alcohol ethoxylates, caused a range of damage ...
Tissues, embryos and pups were fixed in 4% paraformaldehyde, processed routinely in alcohol and xylene, embedded in paraffin, sectioned at 5-micron thickness, and stained with hematoxylin-eosin (H&E). Multiple sections were obtained through the head in the coronal plane, trunk in the transverse...