Co-stimulatory molecule upregulation andGFP expressionin DCs were quantified using an FC500 flow cytometer (Beckman Coulter, Brea, CA, USA), and data were analyzed usingFlowJosoftware (Tree Star/Becton, Dickinson and Company, Franklin Lakes, NJ, USA). Images ofGFPexpression in cultured cells we...
IPP and DMAPP contents were determined by the specific ELISA Kit from the Sci-tech innovation company (Qingdao, China). Baed on the manufacturer’s instructions, each sample was assayed five times. Subcellular Localization To investigate the subcellular localization of LAS1, we amplified the full-...
have a significant financial interest in Vir Biotechnology, Inc., a company that may have a financial interest in the results of this research and technology. This potential individual and institutional conflict of interest has been reviewed and managed by O.H.S.U. The remaining authors declare ...
MSz is a shareholder in a spin-off company founded to develop AOX-based therapeutics. Acknowledgments We thank Tea Tuomela for technical assistance and Aapo Kiviniemi for advice on legal matters. Appendix A. Supplementary data Download all supplementary files included with this articleWhat’s this?
(Gelcompany). In-gel proteolytic cleavage with sequencing grade trypsin (Promega) was performed as described [40]. The peptides from the digestion were dried and redissolved in 0.05% trifluoroacetic acid, 2% acetonitrile for analysis with liquid chromatography coupled with tandem mass spectrometry. ...
SS holds a patent related to the manuscript content but has never received reimbursements, fees, funding, or salary from a company related to the manuscript content. Authors’ contributions M. Ueki contributed to the initial conception of the study and participated in its design. M. Ueki, M. ...
(F) Boxplots showing changes in gene expression levels upon SARS-CoV-2 infection, as quantified by RNA-seq, for all expressed genes (Exp), TOP1-dependent induced genes (Dep), and TOP1-independent induced genes (Indep). (G) Violin plots showing changes in PC1 (delta PC1) at 8 and ...
Ultraviolet radiation (UVR) is a major environmental genotoxic agent. In skin, it can lead to the formation of mutagenic DNA damage. Several mechanisms are in place to prevent the conversion of these DNA damage into skin cancer-driver mutations. An impor
areas. MDA (nmoles /mL/mg of protein) levels in the different brain regions (cerebellum, cerebral cortex, cerebral hemispheres and medulla) of animals treated with permethrin (PERM 150 and 300 mg/kg) are shown as percentages (mean ± SD) of the levels detected in the vehicle and sham ...
then the suppressive power of the drive may be lost if drive conversion is modest. Avoiding this could involve alternate stronger tTAV prompters or utilizing genomic insertion sites that allow for higher transgene expression. However, we found that small levels of female heterozygote survival actually...