结果: (1)24h和48h,ISK-let-7e-5p组let-7e-5p的mRNA表达量都显著增加,CDKN1A的mRNA相应降低,说明过表达hsa-let-7e-5p可在mRNA水平抑制靶基因CDKN1A的表达,而且24h组的过表达效果较好,则选定最佳转染时间为24h,Real-timePCR鉴定let-7e-5pmimics转染成功.p<0.05,差异有统计学意义. (2)荧光显微镜下观察细胞...
本发明通过实验证实,miR‑125a‑5p和let‑7e‑5p共同靶向PRKAA2基因通过AMPK途径促进非酒精性脂肪肝病进展,而miR‑125a‑5plet‑7e‑5p拮抗剂可以减缓非酒精性脂肪肝病进展,在治疗非酒精性脂肪肝病中具有很好的应用前景。 专利权项:1.一种miRNA拮抗剂组合物,其特征在于:该组合物包括miR-125a-5p拮抗...
The expression of let-7e-5p and levels of IFN-纬 and IL-12 were increased, while the expression of Fas and FasL and levels of IgE, sIgE, IL-4 and IL-13 were decreased (all P< 0.05). Compared with the AR antagomir-NC group, the allergic rhinitis score was increased in the ...
目的:探讨let-7e-5p对变应性鼻炎(AR)小鼠调节性T细胞(Treg)/辅助性T细胞17(Th17)失衡的影响.方法:12只BALB/c小鼠,3只为正常组,余9只小鼠采用卵清蛋白(OVA)和氢氧化铝[Al(OH)3]建立AR模型后随机均分为模型组(仅造模),let-7e-5p激动剂对照组(鼻内滴注let-7e-5p agomir阴性对照)及let-7e-5p激动剂组(...
FASL1型辅助性T细胞2型辅助性T细胞目的探讨let-7e-5p对小鼠变应性鼻炎的影响及机制.方法将18只BALB/c小鼠按随机数字表法均分为对照(Control)组,变应性鼻炎(AR)组,let-7e-5p激动剂阴性对照(AR+agomir-NC)组,let-7e-5p激动剂(AR+agomir)组,let-7e-5p抑制剂阴性对照(AR+antagomir-NC)组和let-7e-5p抑制...
In this study, we aimed to assess the prognostic value of these highly controversial miRNAs, miR-375-3p, miR-210-3p and let-7e-5p in breast cancer tissues by investigating their expression association with patients’ pathological response to neoadjuvant therapy and clinicopathological features. 2. ...
Figure 1. The difference of microRNA between remission and non-remission in patients with major depressive disorder: (a) let-7e; (b) miR-21-5p; (c) miR-223 (d) miR-145; (e) miR-146a; (f) miR-155. Before antidepressants treatment, let-7e, miR-21-5p, miR-145, and miR-146a we...
MicroRNA hsa‐let‐7e‐5p in hUC‐MSC‐EVs alleviates oral mucositis by targeting TAB2doi:10.1111/sji.13399Lin, ShutingLai, DongTian, YanLai, FeiLong, MinJi, ChangfuHao, GengxinScandinavian Journal of Immunology
Hsa-let-7e-5p expression was significantly down-regulated in HNSCC tissues and highly metastatic PCI-37B cells. Bioinformatic analysis predicted that hsa-let-7e-5p bound to the 3'untranslated region(3'UTR) of chemokine receptor 7(CCR7), which was validated by luciferase assay. While transfection...
In conclusion, our data suggest that increased LINC00894 expression reduces the oncogenic properties of thyroid cancer cells by sponging let-7e-5p to promote TIA-1 expression.Bo ChenDeqing LiuRunjie ChenLibing GuoJianmin RanHormones and Cancer...