Let-7a-5p富集于hucMSCs-exo中,且hucMSCs-exolet-7a-5pmimic对CVB3诱导的铁死亡的抑制作用高于hucMSCs-exomimic NC。在VMC组中,SMAD2表达升高,而ZFP36表达降低。Let-7a-5p靶向SMAD2信使RNA(mRNA),且SMAD2蛋白与ZFP36蛋白直接相互...
7a in breast cancer cells. Deregulation of miR-let-7a-5p is associated with oncological diseases. Thus, miR-let-7a acts as a tumor suppressor and is down-regulated in many types of cancer. Reduced levels of miR-let-7a-5p were reported in lung squamous carcinoma, colorectal cancer, ...
说明:tch-let-7a-5p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录产物的翻译,从而起到转录后调控靶基因的表达。 miRNAs广泛存在于动物、植物、真菌...
目的探讨let-7a-5p靶向调节转化生长因子一β受体1(transforming growth factor-β receptor 1,TGF-βR1)在急性呼吸窘迫综合征中作用及机制.方法 NIH3T3细胞经转染let-7a-5p pmimics或let-7a-5p inhibitor等后,分为1et-7a-5p增强组,let-7a-5p增强对照组,let...
本发明公开了人脐带间充质干细胞外泌体中let‑7a‑5p的用途,涉及生物医药技术领域,其技术要点为:本发明将人脐带间充质干细胞外泌体中的let‑7a‑5p应用在制备治疗病毒性心肌炎的药物中;所述外泌体中let‑7a‑5p通过介导SMAD2以促进ZFP36表达的途径抑制CVB3诱导的心肌细胞铁死亡,实现对病毒性心肌炎的治...
本试验的目的是分离和培养具有多向分化潜能的鸡BMSCs,探究gga-let-7a-5p靶向GDF6对BMSCs多向分化的调控作用,为gga-let-7a-5p调控BMSCs在再生医学上的应用和疾病治疗提供实验基础与参考依据. 1,鸡BMSCs分离培养及其多向分化潜能验证.利用全骨髓贴壁法从孵育16-18天鸡胚中获取BMSCs,经过多次更换培养液与控制消化...
Let-7a-5p blocks GLUT12 mediated-tumor growth, metastasis and glycolytic function. • Let-7a-5p expression inversely correlates with GLUT12 expression in TNBC patients. Abstract Triple-negative breast cancer (TNBC) is known for its aggressive phenotype with limited treatment modalities and poor prog...
Further, we found that the crosstalk between BCL-xL and let-7a-5p could lead to toxic autophagy and cell death through activating the PI3K-signaling pathway, which was independent of apoptosis or pyroptosis. These findings indicate that let-7a-5p is a sensitive initiator for toxic autophagy in...
At multivariate analysis, only let-7a-5p was significantly associated with EFS (p = 0.009) and OS (p = 0.0008). Conclusion: Up-regulation of the above miRNAs could represent biomarkers in breast cancer. 展开 关键词: subtypes breast cancer miRNAs breast cancer treatment chemotherapy integrated ...