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The supernatants were transferred to new tubes, and to each one 0.5 mL of isopropanol was added. They were then incubated for 10 min and centrifuged at 12,000× g for 10 min at 4 ◦C. The supernatants were discarded, and the RNAs were washed with 1 mL 75% ethanol. The pellets ...