GMP Lentiviral Vectors Production Lentivirus Integration Site Analysis Service Custom CRISPR Lentiviral Library Replication-Competent Lentivirus Testing Custom CAR Lentiviral Service Lentivirus Testing Services Lentiviruses (LVs) are an attractive gene delivery vehicle for a wide broad of applications. LVs offer...
Of note, the overall levels of integration and infection are reduced in these non-dividing host cells compared with their dividing counterparts, suggesting that lentiviruses may prefer the actively replicating sub-population of cells but nevertheless have preserved the ability to enter quiescent cells. ...
Thelentivirusis a subclass of retrovirus. As such, infection is associated with permanent integration of the gene, and thus can affect nondividing cells and terminally differentiated cells such as neurons, macrophages, and hematopoietic stem cells (Barker and Planelles, 2003). They have broad tissue...
An integration-defective lentivirus-based resource for site-specific targeting of an edited safe-harbour locus in the human genome 来自 Semantic Scholar 喜欢 0 阅读量: 56 作者: R Torres,A Garcia,M Jimenez,S Rodriguez,JC Ramirez 展开 摘要: Optimized gene transfer into human cells are ...
Therefore, LV integration pattern in genomic DNA obtained from day 7 SmartDC-TRP2 was analyzed by linear amplification mediated-PCR (LAM-PCR) and next-generation sequencing and the clonal frequencies were monitored with a high-throughput integration site (IS) analysis.31 The number of total ...
An integration-defective lentivirus-based resource for site- specific targeting of an edited safe-harbour locus in the human genome. Gene Ther. 2014; 21:343-352.An integration-defective lentivirus-based resourse for site-specific targeting of an edited safe-harbor locus in the human genome. ...
Inverse PCR strategy for integration site analysis demonstrated integration of at least 1 or 2 copies of GFP in the transgenics, mapping to different chromosomes. GFP expression was detected in a wide range of murine tissues, including testis and the transgene was stably transmitted to a third ...
293T cells were transduced with VSV-G pseudotyped vector particles containing either the wild type HIV-1 integrase or its E152A mutant. Earlier studies which characterize this mutant in the context of the HIV-1 virus demonstrated a complete loss of integration activity while all other steps in...
Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal ...
Being a monogenic disorder, FRDA is amenable to LV gene therapy, a strategy that offers permanent FXN gene delivery and integration into the host genome. We used LVs containing the SFFV promoter not only to determine whether LVs could correct FRDA at the molecular level, but also to ...