(2012). Lentivector transduction improves outcomes over transplantation of human HSCs alone in NOD/SCID/Fabry mice. Mol. Ther. 20, 1454-1461. doi: 10.1038/mt.2012.64Pacienza N, Yoshimitsu M, Mizue N, et al. Lentivector transduction improves outcomes over transplantation of human HSCs alone in ...
These lentivectors benefit from the advantages of efficient transduction of nondividing cells, low natural antivector immunity, and a low potential for genotoxicity due to insertional mutagenesis. Numerous modifications of the lentivector have improved their safety profile and ability to elicit a strong ...
The invention provides a method for making a mammalian cell that produces a protein by transducing a mammalian cell with lentiviral transduction vectors at least 2 times in 24 hours, wherein each lentiviral transduction vector comprises an expressible polynucleotide coding for the protein. In one ...
Subject:Molecular Cell Biology Subject:Signal Transduction Subject:Signaling in Selected Disciplines Subject:Developmental Signaling Subject:Neuroscience Subject:Developmental Neuroscience ScienceOpen disciplines:
Packaging HIV- or FIV-based lentivector expression constructs and transduction of VSV-G pseudotyped viral particles. As with standard plasmid vectors, it is possible to transfect lentivectors in plasmid form into cells with low-to-medium efficiency to obtain transient exp... A Mendenhall,J Lesnik...
Here we demonstrate that the relative efficiency of cell-cell surface transmission can outpace "cell-free" transduction at limiting vector input. This coincides with the prolonged half-life of cell bound vector but occurs, unlike HTLV-1, without evidence for particle aggregation. These studies ...
This platform, termed DCVex(TM), utilizes a novel genetic variant of a Sindbis virus envelope glycoprotein with post-translational carbohydrate modifications in combination with Vpx, a SIVmac viral accessory protein, to achieve efficient targeting and transduction of DCs. In addition, DCVex(TM) ...
While the pLL-CMV-GFP-T2A-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of targ...
Frecha C,Lévy C,Cosset FL,et al.Advances in the field oflentivector-based transduction of T and B lymphocytes forgene therapy. Molecular Therapy . 2010Frecha, C., Levy, C., Cosset, F. L., and Verhoeyen, E. (2010). Advances in the field of lentivector-based transduction of T and ...
Different degrees of transduction observed in differentiated and undifferentiated cells might be the result of various lentiviral receptors on the plasma membrane, site of integration, and activity of CMV promoter. Further, other regulatory factors involved in cell transduction and target gene expression ...