摘要: The length of DNA strands is an important resource in DNA computing. We show how to decrease strand lengths in known molecular algorithms for some NP-complete problems, such as like 3-SAT and Independent Set, without substantially increasing their running time or volume....
Under single turnover conditions in the presence of excess enzyme, the quantity of DNA unwound increased significantly as the length of the single strand overhang increased. Increased processivity was observed when the DNA substrate contained longer single-stranded overhangs. Equilibrium binding studies ...
The present application discloses a method and kit for adjusting the length of an overhang formed at a DNA end by using competitive inhibition, and dsDNA linkage method using the same. The ligase-independent DNA linkage method using the method and kit according to the present application has ...
-x=<min>:<max>strand shifts to exclude (This is mainly to avoid region around phantom peak) default=10:(readlen+10) -p=<nodes>number of parallel processing nodes, default=0 -fdr=<falseDisoveryRate>false discovery rate threshold for peak calling ...
Small library size can be the result of RNA degradation due to either contamination (e.g. RNases), apoptosis, inefficient transcript capture before first strand synthesis or cDNA amplification. Importantly for our comparison, library size may also depend on protocol chemistry, reflecting the ability...
The distribution of lengths of single-strand DNA in Chinese hamster ovary cells in the G1 phase of the cell cycle has been observed for various conditions of cell lysis and incubation of the lysates. The method of analysis was band sedimentation through a self-generating density gradient, a te...
The chain termination DNA sequencing procedure of Sanger et al. (1977) requires single-stranded DNA as template. M13 phage DNA exists as a single strand an... PH Schreier,R Cortese - 《Journal of Molecular Biology》 被引量: 395发表: 1979年 Cooperative interaction between Ca2+ binding sites ...
Single Strand Conformational Polymorphism (SSCP) of a 300 bp DNA fragment, from exon 9 of samples from an egg laying North American commercial White Leghorn stock, revealed a total of 6 different single strand conformers, indicative of 3 alleles. Subsequent DNA sequencing found a total of 4 ...
First-strand synthesis of cDNA was performed using RR047 kit (Takara) (1 μg of total RNA was used for cDNA synthesis in a 20 μl reaction volume). In order to adjust the PCR reaction for flavonoid biosynthesis gene, the amount of the template was adjusted, and then the semi-...
(-34...-37) and T5(-34...-38) tracts located in the nontemplate DNA strand had the largest and comparable effect on the promoter strength, while the A5.T5(-37... -41) sequence in either orientation (A5 tract in the template or nontemplate strand) exerted a much smaller effect. ...