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Stationary promastigotes were grown at 24 °C in Schneider’s medium (Sigma, St. Louis, MO, USA), which was supplemented with 20% inactivated fetal bovine serum (FBS, Sigma, St. Louis, MO, USA), 20 mM L-glutamine, 200 U/mL penicillin, and 100 µg/mL streptomycin, at pH 7.4. ...