We isolated LPS-insensitive mutants defective in the bulb-type lectin S-domain-1 receptor-like kinase LORE (SD1-29), which were hypersusceptible to infection with Pseudomonas syringae. Targeted chemical degradation of LPS from Pseudomonas species suggested that LORE detected mainly the lipid A moiety...
Plants have a vast array of pattern-recognition receptor to detect microbial ligands, but how they sense lipopolysaccharide has remained unknown. Ranf et al. identify the receptor-like kinase SD1-29 as a de facto pattern-recognition receptor for lipopoly
This immune response includes the activation of highly conserved signaling cascades such as the p38 MAP kinase pathway, the programmed cell death pathway (PCD), the transforming growth factor-β (TGF-β) pathway, the insulin-like receptor (ILR), transcription factors (ZIP-2, DAF-16, SKN-1) ...
Soluble TMD1 binds to Lewis Y antigen on the LPS, thereby preventing engagement of LPS, CD14, and TLR4, resulting in reduced activity of NFκB and mitogen activated kinase/ERK pathways. LeY, Lewis Y antigen; TLR4, toll-like receptor 4; NFκB, nuclear factor κB; ERK, extracellular signal...
Lectin-like domain; LPS: Lipopolysaccharides; MAPK: Mitogen-activated protein kinase; MEFs: Mouse embryonic fibroblasts; MFI: Mean fluorescence intensity; MOI: Multiplicity of infection; mTOR: Mammalian target of rapamycin; Mx1: MX dynamin-like GTPase 1; PFU: Plaque forming unites; dpi: Days post...
Even when the actual ligand that triggers this receptor is not known, cross-linking of CD69 by specific antibodies activates the extracellular signal-regulated kinase signaling pathway (23) and has been shown to induce rise in intracellular calcium concentration, synthesis of different cytokines and/...
Receptor kinaseA novel gene encoding a -like was cloned from the () through cDNA library screening. This gene (named as Ghlecrk; Gen: AY487461) had a total length of 2233bp with an open reading frame of 1926bp, and encoded a predicted of 641 amino acids with a molecular weight of ...
kinase MPO = myeloperoxidase NF-κB = nuclear factor-κB PAMPs = pathogen-associated molecular pattern molecules PBS = phosphate-buffered saline PCR = polymerase chain reaction TEM1D1 = TEM1 lectin-like domain TLR = toll-like receptor TNF-α = tumor necrosis...
This probe was 5′-end radiolabeled using T4 polynucleotide kinase (CLONTECH) and [γ-32P]ATP (ICN Biomedicals). Membrane prehybridization was carried out at 60 °C for 3 h in 20 mm phosphate buffer, pH 7.5, containing 5 × SSC, 7% SDS, 10 × Denhardt's solution, and 1% salmon ...
Nishiguchi M, Yoshida Y, Sumizono T et al. A receptor-like protein kinase with a lectin-like domain from Lombardy poplar: gene expression in response to wounding and characterization of phosphorylation activity. Mol Genet Genom 2002; 267:506–514....