For the toxicity test, normalization of each group was carried out using the mean of the untreated cancer cell line. We employed the Abcam Bacterial Viability Assay Kit (ab189818). Bacterial cultures were grown overnight and harvested by centrifugation at 10,000 x g for 10 min. The cells ...
Leaky gut syndrome happens when the epithelium on the villi of the tight junctions of the small intestine becomes inflamed and weakened, which allows toxins, bacteria,…
Overview of the GAPS Intro Stages with allowed food and an explanation of how the GAPS Introduction Diet specifically heals and seals the gut lining.
Leaky gut and leaky brain often occur at the same time. This is not surprising if you consider the connection between your gut and your brain. The proteins occludin and zonulin can both lead to gut and blood-brain barrier permeability. If you have antibodies against these two proteins, it m...
Unpaired Student’s t-test was performed in (B–D). Ordinary one-way ANOVA was performed in (E). * p < 0.05 and ** p < 0.01 relative to corresponding 2 w or 12 w untreated mice. As we had previously observed that a bacterial infection promotes aSyn oligomerization in the gut [15...