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The abaxial surface of leaf was brushed by nail polish and a fine transparent negative film of the epidermis was obtained. The picture of epidermis was observed under microscope and captured by a camera. The length of two kinds of epidermal cells (long cells and interstomatal cells) are ...
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The transparent samples were dehydrated in gradual ascending ethanol series and stained with a 1% alcoholic safranin solution (Sigma). The samples were observed under a Leica MDG 34 stereomicroscope and recorded with a Leica DFC 425 digital camera. The surface of the leaf glands was examined using...