叶盘转化法-leaf disc transformation Leaf Disk Method For Agrobacterium Mediated Transformation of Higher Plants Background The gram negative soil bacterium Agrobacterium tumefaciens causes crown gall disease in plants. This disease is characterized by the formation of tumors on plants after infection of ...
. 2008 . Establishment of a highly efficient Agrobacterium tumefaciens -mediated leaf disc transformation method for Broussonetia papyrifera . Plant Cell Tiss. Organ Cult . 93 : 249 – 255 .Li M, Li H, Jiang H, Wu G (2008) Establishment of a highly efficient Agrobacterium tumefaciens -...
Leaf Disk Transformation Abstract Reliable and efficient methods of transferring cloned genes into plants are essential for engineering crops with desired traits. The Gram-negative soil bacteria,Agrobacterium tumefaciensandA. rhizogenes, are natural genetic engineers, capable of transforming a range of dicoty...
[25]. The Chinese Academy of Sciences Institute ofMicrobiologysuccessfully transformed tobacco G2140 byAgrobacterium-infected leaf disc method, and also received transformed tobacco plants and their offspring of hepatitis B immune activity. Purified HBsAg in theelectron microscopeexamination also presented ...
These vectors were transformed into yeast strain YM4271 using the PEG/LiAc method, and yeast cells were incubated in SD/-His/-Leu liquid medium. β-Galactosidase activity was determined by absorbance of chloramphenicol red, a hydrolysis product of chlorophenol red-β-D-galactopyranoside (CPRG), ...
because the multigene transformation using agroinfiltration does not require selection markers, which are normally necessary for the regeneration of stably transformed plantlets. This is also a great advantage of agroinfiltration. Another way to obtain multigene expression is a method in which leaves of ...
II. Preliminary observations using the disc-culture method. Ann. Bot.6: 455–468. 1942. CAS Google Scholar Mathuse, O. Ueber abnormales, sekundäres Wachstum von Laubblättern, insbesondere von Blattstecklingen dicotyleen Pflanzen. Beit. Bot. Zentralbl.20: (174) 1–44. 1906. Google ...
the occurrence of transformation can be confirmed. In addition, an amplification product can be detected by PCR using primers preliminarily labeled with a fluorescence dye or the like. Further, it is also possible to carry out a method comprising allowing an amplification product to bind to a so...
1) Leaf disc transformation 叶圆片转化2) Foliar invertases 叶片转化酶3) Circule 叶片圆环4) blade tip rounding 修圆叶片 1. Numerical simulation was performed for these schemes of axial pump s clearance flow by using software FLUENT,and the patterns of pressure and velocity distributions in ...
3) leaf-disc transformation 叶盘转化 1. Throughleaf-disc transformationmethod, regenerated transgenic tobacco seedlings with AFP gene were obtained. 通过叶盘转化方法得到了再生的AFP转基因烟草苗;利用授粉后花粉管通道技术将AFP基因导入栽培棉花。 4) wire melting rate ...