Elevated LDL concentrations in blood and anincrease in their residence time coupled with an increase in the biologicalmodification rate results in the destruction of the endothelial function and ahigher LDL-cholesterol uptake in the monocyte/macrophage system as well asby smooth muscle cells in vessel...
Luciferase reporter assay and site-directed mutagenesis identified that hsa-miR-140-5p interacts with the predicted seed sequence "AAACCACU" in the 3'-UTR of human LDLR mRNA. Hsa-miR-140-5p over-expression attenuated LDL-C uptake and decreased intracellular cholesterol levels in the presence of...
In a recent study, we reported that rapamycin inhibits ox-LDL uptake in HUVECs. In this study, we showed that rapamycin inhibits ox-LDL-stimulated adhesion molecule expression and macrophage adhesion to HUVECs. Whether rapamycin has an effect on other processes of atherosclerosis warrants further ...
In our previous studies, we reported that the uptake of oxidized low density lipoproteins (OxLDLs) is reduced by approximately 50% in Mphi from CD36-deficient patients compared with that in control subjects. Recently, we have shown that CD36 is highly expressed in human atherosclerotic aorta. ...
This number of substituted lysines corresponds to the minimum number required for scavenger-mediated uptake of oxidized LDL. Substituting aldehydes can be produced by peroxidation of lipids of LDL, which results in the generation of oxidized LDL. However, lipid peroxidation is not required. Indeed, ...
Plasma blood urea nitrogen levels were determined using a colorimetric assay (Bioquart, San Diego, CA). Plasma glucose levels were measured using the ACE® clinical chemistry system (Alfa Wassermann, Inc., West Caldwell, NJ). Plasma lipoprotein and lipid levels were determined as described previo...
Rapamycin and its derivative possess anti-atherosclerosis activity, but its effects on adhesion molecule expression and macrophage adhesion to endothelial cells during atherosclerosis remain unclear. In this study we explored the effects of rapamycin on ox-LDL-induced adhesion molecule expression and macroph...