货号:CK12,完美替代promega:G1780,性价比高。 Cytotoxicity LDH Assay Kit-WST®是通过测定细胞释放到培养基中的乳酸脱氢酶(LDH)活性进而测定细胞损害的试剂盒。LDH(乳酸脱氢酶)是存在于细胞质的一种酶,当细胞膜受到损伤时,LDH 会释放到培养基中。由于释放出的LDH 稳定,检测培养基中LDH 的量可以作为测定死细胞...
碧云天的乳酸脱氢酶细胞毒性检测试剂盒(LDH Cytotoxicity Assay Kit),也称乳酸脱氢酶检测试剂盒(LDH Assay Kit)或乳酸脱氢酶释放检测试剂盒(LDH Release Assay Kit),是一种基于diaphorase催化的INT显色反应,通过比色法检测细胞毒性时释放的乳酸脱氢酶活性或检测其它样品中的乳酸脱氢酶活性的试剂盒。 本试剂盒可以用于常...
货号:CK12,完美替代promega:G1780,性价比高。 名称 品牌 货号 规格 单位 价格 LDH/死细胞检测/细胞毒性检测 日本同仁 CK12 500T 盒 1610 LDH/死细胞检测/细胞毒性检测 日本同仁 CK12 2000T 盒 3680 CCK8/MTT法替代品/细胞增殖检测 日本同仁 CK04 500T 盒 890 Cytotoxicity LDH Assay Kit-WST®是通过测...
Cytotoxicity LDH Assay Kit-WST®是通过测定细胞释放到培养基中的乳酸脱氢酶 (LDH) 活性进而测定细胞损害的试剂盒。LDH是存在于细胞质的一种酶,当细胞膜受到损伤时,LDH会释放到培养基中。由于释放出的LDH稳定,检 测培养基中LDH量可以作为测定死细胞和受损细胞数量的指标。本试剂盒可以测定受损细胞所释放出的LDH...
CellTiter-Glo Promega Cat. # G7571 NAD/NADH Quantitation kit Sigma-Aldrich Cat. # MAK037-1KT Lactate Colorimetric/Fluorometric Assay Kit Abcam Cat. # ab65330 Seahorse XF Cell Mito Stress Test Kit Agilent Cat. # 103015-100 Experimental Models: Cell Lines MIA PaCa-2 ATCC Cat. # ATCC® CR...
Lactate assay: Lactate concentrations were measured using a lactate assay kit (MAK064, Sigma Aldrich, UK) according to the manufacturer's instructions. Cells were lysed and protein concentration determined by BCA. 20 µL of lysates were analysed at 570 nm using POLARstar OPTIMA plate reader (...
Fourthly, double-fluorescence values were detected using the SYNERGY HTX multi- mode reader (BioTek, Winooski, VT, USA) and Dual-Luciferase kit (Promega, Madison, WI, USA) after transfection for 48 h. For identifying the specific binding sites of transcription factor HIF-1, we performed ...