performance and stability.Test principleUV assayLactate dehydrogenase catalyzes the conversion of L-lactate to pyruvate;NAD is reduced to NADH in the process.LDHL-Lactate + NAD + Pyruvate + NADH + H +The initial rate of the NADH formation is directly proportionalto the catalytic LDH activity. ...
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humid...
LDH-A silencing also failed to induce p21 in ARPE19 cells, consistent with no effect on SIRT1 activity or p53 K382 deacetylation (Figures 4b and c). These results provide proof-of-principle that suppressing LDH-A can provide a way of altering the activity of non-glycolytic NAD þ -...
.roche-applied-science CytotoxicityDetectionKit (LDH) yVersion08 Contentversion:February2011 Anon-radioactivealternativetothe[ 3 H]-thymidinereleaseassayandthe [ 51 Cr]-releaseassay. Colorimetricassayforthequantificationofcelldeathandcelllysis,basedonthe measurementoflactatedehydrogenase(LDH)activityreleasedfrom...
Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate,with the concomitant interconversion of NADH and NAD+.Intended Use:Measurement of released LDH in culture mediumEvaluation of ...
2) LDH release assay LDH释放法 1. Using LDH release assay to detect cytopathic extent and determine antiviral activity of interferons; 用LDH释放法判断细胞病变程度确定干扰素生物活性3) LDH release LDH释放4) LDH assay LDH释放实验5) LDH releasing test LDH释放试验 1. To set up method of ...
As shown in Figure 2, the resazurin assay revealed a slight decrease in the metabolic activity upon 48 h of exposure to LDH at 25 µg/mL. However, this effect was not statistically significant versus the experimental control (cells treated with the vehicle alone). Remarkably, LDH did not ...
The HCG Pregnancy Rapid Test Midstream is a rapid one step assay designed for qualitative detection of human chorionic gonado (HCG) in urine. The test midstream can accurately detect pregnancy when the level of HCG is between 25mIU/ml to 500,000mIU/ml. [PRINCIPLE] Because...