Furthermore, SphK2/ CD4+ T cells were able to promote endogenous, virus-specific CD8+ T cell responses. Importantly, oral treatment of LCMV Cl 13-infected mice with an SphK2-selective inhibitor increased the number of LCMV-reactive CD4+ and CD8+ T cells, and led to the accelerated ...
Results LCMV-CL13-infected mice maintained a tissue viral load of 1×106 copies/ μL virus. The percentage of CD4+ T cells in the peripheral blood gradually increased in the infection plateau phase to ( 13. 15% ± 0. 72%), while the percentage of CD8+ T cells first decreased to ( ...
The immunosuppressive LCMV isolates WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a generalized immunosuppression of the host allowing viral persistence. These ...
Spleen samples were depleted of CD4 and B cells by incubating splenocyte suspensions in enrichment buffer (PBS, 1% FCS, 2 mM EDTA) with biotinylated α-CD4 and α-B220 antibodies (1/100 dilution) at room temperature for 20 min, followed by incubation with streptavidin-conjugated beads ...
GITR-/- mice have defective follicular helper CD4 T cell responses following LCMV cl 13 infection.doi:10.1371/JOURNAL.PPAT.1004517.G005Derek L. ClouthierA. ZhouM. WortzmanOlga LuftG. LevyT. WattsPLOS Pathogens
DCs from Cl13 infected mice induce IL-10 expression in naïve LCMV specific CD4 T cells.Carmen Baca JonesChristophe FilippiSowbarnika SachithananthamTeresa RodríguezCalvoKatrin EhrhardtMatthias von Herrath
Virus dose LCMV cl-13 Persistent infection CD4+ T cells Wasting Cachexia TNFα IFNγ Introduction Individuals infected with viruses experience a loss in their quality of life. This loss may be short and transient, such as following an influenza virus infection, or may be life-long, as occurs...