Here, we map selective expression profiles of dopamine neurons in an extensive collection of human SNc and VTA using laser capture microdissection coupled with Smart-seq2 RNA sequencing (LCM-seq). By applying a bootstrapping strategy as sample input to DESeq2, we identify 33 differentially ...
RNA was extracted by RNeasy Plus Micro Kit (Qiagen) and, following quality control steps, cDNA libraries constructed (SMART-Seq v4 Ultra Low Input RNA Kit; Takara Bio) and sequenced (NovaSeq 6000, Illumina) to obtain 150 bp strand-specific paired-end reads at ~ 33 million reads/sample. ...