LC3-II 的分子量大于LC3-I,分子间存在极强的疏水作用力,SDS-PAGE 电泳时LC3-II 涌动的速度比LC3-I 快,所以可以检测到两条带,上面的条带为LC3-I,下面的条带为LC3-II,典型的western 结果如下:通过western blot 检测ATG12-ATG5 和LC3 的水平,判断机体的自噬现象。3Weste...
LC3是自噬形成标志物。 P62与经泛素化的蛋白结合,再与LC3-II蛋白结合形成复合物,并最终在溶酶体内降解,在自噬过程中P62会不断被消耗。 这三个蛋白是检测自噬的关键蛋白。 Frontiers in immunology 9 (2018): 1881. 应用Immunoway LC3B (YM3381) 其他检测方法...
因此可以通过LC3-II/I比值的大小估计自噬水平的高低。 图8. WB检测LC3、p62蛋白的表达(Yoshii, S.R. and N. Mizushima. Int J Mol Sci, 2017) ②除LC3外,其他自噬底物表达量的变化也可以用于监测自噬流。其中,p62是研究广泛的一个自噬底物。在自噬体形成过程中,p62作为链接LC3和聚泛素化蛋白之间的桥梁,被...
2.Phagophore 不断延伸,LC3B逐渐募集,p62随后带着需要降解的货物结合上来,磷脂双层膜将胞浆中的任何成分,包括细胞器,全部揽入“碗”中,然后“收口”,成为密闭的球状的autophagosome,即“自噬体”。电镜下观察到自噬体是自噬发生的铁证之二。有两个特征:是双层膜,二是内含胞浆成分,如线粒体、内质网碎片等。 3....
结果显示,雷帕霉素增强LC3I向LC3II转化,促进p62降 解,而海藻糖仅引起LC3II表达的上调;阻断细胞自噬过程各个阶段均导致LC3II和 p62堆积,并呈现出剂量和时间依赖 性。这些结果提示尽管LC3II和p62均为自噬标记物,但不同的调节剂对其表达的调控存在差异。 关键词 自噬活化剂;自噬抑制剂;LC3II;p62蛋白 中图分类号 R...
Inhibition of apoptosis increased the conversion of LC3-I to LC3-II and degradation of P62.XueYan, XiXiaoyan, ZhangBei, WangTao, WangJi, WangHe, HuangJianwei, WangQi, JinZhendong, Zhao
Beclin-1蛋白表达及LC3-II/LC3-I比值下降(P<0.01),p62蛋白表达升高(P<0.01),溶酶体荧光强度降低(P<0.01),ASMase的荧光强度升高(P<0.01),ASMase与LAMP1的... 李银萍,宣悦,廖玮嫣,... - 中药新药与临床药理 被引量:...
However, the co-localization phenomenon of NSs with LC3, P62 and Lamp2b proteins disappeared in the NSs-GFP transfected cells. Previous studies reported the activation of autophagy upon virus infection as inferred from the increased number of autophagic structures and the monitoring of LC3-I to ...
(LC3II)/I ratio, and decrease p62 protein level compared with control cells upon radiation in HCT116 cells (Fig.3D, Eand Supplementary Fig.S3a). Increased LC3II/I ratio and decreased p62 protein level were also found in A549 PDI knockdown cells. Moreover, the number of green fluorescent ...
LC3ILC3IIP62BACKGROUND: Using neural stem cells (NSCs) in cell therapy and regenerative medicine is a growing knowledge. In this study, the protective role of carnosic acid and trehalose against H2O2-induced oxidative stress in autophagy induction and apoptosis inhibition in NSCs was investigated. ...