LCYW-EN181(UR)Bottomless Trap Hole(奈落的落穴)LCYW-EN182(UR)Royal Decree(王宫的通告)LCYW-EN183(UR)Gravekeeper's Spy(守墓的侦察者)LCYW-EN184(UR)Gravekeeper's Guard(守墓的番兵)LCYW-EN185(UR)Gravekeeper's Spear Soldier(守墓的长枪兵)LCYW-EN186 Gravekeeper's Watcher(守墓的监视者)LCYW-...
2C). LC3-trap Yx2 did not pull down any of these proteins under the tested conditions. However, under the explored conditions expression levels of LC3C protein was not detectable in ZBR cells by Western blot in any fraction (Fig. S4). Importantly, ubiquitin was not found in the bound ...
8f). Since we identified KIF11 as the only proteome-wide high affinity target and given the established role of this kinesin in cell division, KIF11 inhibition by 3 might trap cells in mitosis preventing progression of cell division31. Therefore, we analyzed the images taken during the live ...
LCYW-EN181(UR)Bottomless Trap Hole(奈落的落穴)LCYW-EN182(UR)Royal Decree(王宫的通告)LCYW-EN183(UR)Gravekeeper's Spy(守墓的侦察者)LCYW-EN184(UR)Gravekeeper's Guard(守墓的番兵)LCYW-EN185(UR)Gravekeeper's Spear Soldier(守墓的长枪兵)LCYW-EN186 Gravekeeper's Watcher(守墓的监视者)LCYW-...
GFP-trap assays HeLa cells were transfected with EGFP-LC3 using Mirus TransIT-2020 transfection reagent and 24hrs later were lysed in lysis buffer (50 mM HEPES, 150 mM NaCl, 1% Triton X-100, 1.5 mM MgCl2, 5 mM EGTA) for 10 min on ice and pelleted for 10 min at 13,...
Supplementary Table 4 Real-time PCR primers. Full size table Supplementary information Supplementary Information Supplementary Information (PDF 2508 kb) About this article Cite this article Martinez, J., Malireddi, R., Lu, Q. et al. RETRACTED ARTICLE: Molecular characterization of LC3-associated pha...
-extendedinto16-bitmemoryaddress •Wheretogo –lookupstartingaddressfromtable;placeinPC •Howtogetback –saveaddressofnextinstruction(currentPC)inR7 CMPE12–Winter2009–Ferguson 10-7 TRAP CMPE12–Winter2009–Ferguson 10-8 Return:RET(JMPR7) •Howdowetransfercontrolbackto instructionfollowingtheTRAP...
(HisTrap HP, Cytiva 17524801). The column was washed with PBS buffers (20 mM phosphate, pH 7.5, 300 mM NaCl, 2 mM BME) without and with 30 mM imidazole, and then eluted with PBS buffer containing 500 mM imidazole. The elute of hAtg3 and its mutants (with purities no ...
Protein complexes were immunoprecipitated using GFP-TRAP, followed by immunoblot. See also Figure S2 and Table S1. Atg16L2, an isoform of Atg16L1, cannot function in autophagy, possibly due to its inability to localize to phagophores (Ishibashi et al., 2011). A region in Atg16L1 (229–...
dwelch67/lc3Public NotificationsYou must be signed in to change notification settings Fork4 Star8 Code Issues master 1Branch0Tags Code Repository files navigation README This is a work in progress... This repository is for learning assembly language programming, and other things using the little...