Scientific answers to FAQs on LC3 and autophagy, including LC3 Western blot troubleshooting, the LC3-I/LC3-II ratio, and using autophagy markers and controls.
Increase of the LC3II/LC3I ratio and LC3II-positive vacuoles by MHY1485.Yeon Ja ChoiYun Jung ParkJi Young ParkHyoung Oh JeongDae Hyun KimYoung Mi HaJi Min KimYu Min SongHyoungSam HeoByung Pal Yu
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The graph shows the change in the LC3II/LC3I ratio from four independent experiments; bars show average ± SD. ∗p < 0.05. (D) WB analysis of Tet-ON M2 WT and ATG4D KO cells stably expressing mCherry-ATG4D or mCherry treated with dox. (E) Quantification of (D). The graph ...
(Fig.3D, Eand Supplementary Fig.S3a). Increased LC3II/I ratio and decreased p62 protein level were also found in A549 PDI knockdown cells. Moreover, the number of green fluorescent punctas was strongly increased under IR treatment in PDI knockdown HCT116 cells (Fig.3F, G). Similarly, ...
The LC3-II/Tubulin ratio was calculated relative to that of control medium (CM). (e) Wild-type MEFs expressing GFP-LC3 were treated as indicated (AMDE-1, 10 μM; 3-MA, 10 mM; Baf, 0.5 μM) for 6 h, and then assessed for LC3 puncta formation. (f) FIP200KO-MEFs expressing ...
Serum apolipoprotein B-to-apolipoprotein A1 ratio is independently associated with disease severity in patients with acute pancreatitis[J]. Sci Rep, 2019,9(1):7764. DOI: 10.1038/s41598-019-44244-w . 返回引文位置Google Scholar 百度学术 万方数据 [4] Miao B , Qi WJ , Zhang SW ,et al. ...
GAPDH gene expression was used as an endogenous control, and the expression level was calculated by using the (2-∆∆Ct) ratio [4]. 2.5. Statistical analyses Each experiment was repeated at least three times. The data were analyzed by two-tailed unpaired Student׳s t-test and ANOVA ...
Moreover, changes in the LC3-IIs/I ratio reflected those in the total proteolytic flux remarkably in both fresh rat hepatocytes and H4-II-E cell lines. Thus, in addition to a sensitive index of macroautophagy, calculating the cytosolic LC3 ratio became an easy and quick quantitative method ...
Dose-dependent titrations using all LC3/GABARAPs yielded assays with good signal-to-noise ratio and resulted in measured KD values for the tracer between 3 and 17 µM across the human Atg8 family. Thus, this displacement assay was suitable for screening and binding affinity determination of...