目的:分离鉴定吗替麦考酚酯(MMF)原料药中的有关物质.方法:采用液相色谱-电喷雾-傅立叶变换离子回旋共振质谱(LC-ESI-FTICRMS/MSn)技术对MMF原料药进行分析,获得主成分及有关物质的高分辨质谱数据(HRMS)和多级质谱数据(MSn),并对有关物质结构进行推断.结果:结合文献数据和质谱裂解规律,从MMF原料药中初步鉴定出6...
linear ion trap 是线性离子阱，属于质量分析器，FTICR是傅里叶变换离子回旋共振。另外MS/MS和MS是有...
High-efficiency on-line microSPE-nanoLC-ESI-FTICR MS analysis of 500 ng of CaM treated with 15-fold molar excess of peroxynitrite. To illustrate the efficiency of the LC separation, mass spectra of different CaM oxiforms were generated from LC-MS data at the indicated elution times in the...
LC-FTICR-MS for targeted proteomics: characterization of tetrahydroquinoline natural product biosynthetic proteins of symbiotic bacteria from macroorganismal assemblies. American Society for Mass Spectrometry Annual Meeting.; Salt Lake City. 2010; [In this poster, the Sherman group describes identifying the...
Estimating probabilities of peptide database identifications to LC-FTICR-MS observations. Proteome Sci. 4:1.Anderson, K. K.; Monroe, M. E.; Daly, D. S. Estimating probabilities of peptide database identifications to LC-FTICR-MS observations. Proteome Sci. 2006, 4, 1....
2. LC-FTICR MS can assist in developing a model for PKS biosynthesis by monitoring ACP active site bound intermediates, which are difficult to monitor. 3. Low resolution LC-qMS can be used in conjunction with a high-resolution, MS/MS instrument (eg. real world concerns about instrument ...
Identification of collagen IV derived danger/alarm signals in insect immunity by nanoLC-FTICR MS. Biol. Chem. 390: 1303-1311, 2009.Altincicek, B.; Berisha, A.; Mukherjee, K.; Spengler, B.; Rommp, A.; Vilcinskas, A. Identification of collagen IV derived danger/ala...
13C labelingCarotenoid metabolismDegree of labelingFTICR-MSIsotopolog profilingLC-MSMass spectrometry (MS)-based metabolite analysis combined with stable isotope labeling offers a powerful tool to study dynamic regulation of metabolic pathways and metabolite fluxes in biological......
Brinkmalm, An online nano-LC-ESI-FTICR-MS method for comprehensive characterization of endogenous fragments from amyloid beta and amyloid precursor protein in human and cat cerebrospinal fluid, J. Mass Spectrom. 47 (2012) 591-603.Brinkmalm G, Portelius E, Ohrfelt A, Mattsson N, Persson R,...
We developed a software suit to detect, annotate and compare metabolite peaks obtained by LC-FT-ICR-MS Detection of the peaks, prediction of the molecular formula of the peaks, and database searching by the accurate mass of the peaks were done in approx. 30 min/sample (in the case of ...